In vivo microdialysis shows differential effects of prenatal protein malnutrition and stress on norepinephrine, dopamine, and serotonin levels in rat orbital frontal cortex.
In vivo microdialysis shows differential effects of prenatal protein malnutrition and stress on norepinephrine, dopamine, and serotonin levels in rat orbital frontal cortex.
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DOI：
10.1037/bne0000479
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发表时间：
2021-10
影响因子：
1.9
通讯作者：
Mokler DJ
中科院分区：
文献类型：
--
作者：
Church NT;Weissner W;Galler JR;Amaral AC;Rosene DL;McGaughy JA;Rushmore RJ;Larrabee E;Mokler DJ
产前蛋白质营养不良（PPM）改变发育中的大脑，包括单胺能系统和注意力的变化。在本研究中，我们使用体内微透析来检测PPM、急性应激和成年大鼠眶外侧额叶皮质（OFC）两个半球的细胞外血清素（5HT）、多巴胺（DA）和去甲肾上腺素（NE）之间的关系。我们假设产前蛋白质营养不良会改变皮层单胺的细胞外浓度。急性约束压力的影响也被评估，因为PPM改变了大脑对压力的反应。我们使用成年雄性Long-Evans大鼠[10只产前营养不良（6%酪蛋白）和10只产前营养良好（25%酪蛋白）]。每隔20分钟从OFC的左右半球采集样品，共采集6小时，并使用HPLC进行定量。采样2小时后，动物暴露于40分钟的约束应激。在两个半球的所有时间点，PPM动物的细胞外NE水平显著高于营养良好的对照组。相比之下，5HT和DA的基线水平在营养组之间没有差异。在应激暴露的前20分钟，与基线水平相比，两个营养组和两个半球的5HT水平升高，但NE或DA水平未升高。这些数据强调了PPM对神经调节系统的影响以及对急性应激反应的变化概况。需要进一步的研究来确定这些基础反应和压力相关反应如何影响认知表现，以及这些差异是否在认知测试中持续存在。
Prenatal protein malnutrition (PPM) alters the developing brain including changes in monoaminergic systems and attention. In the present study, we used in vivo microdialysis to examine the relationship between PPM, acute stress, and extracellular serotonin (5HT), dopamine (DA) and norepinephrine (NE) in both hemispheres of lateral orbital frontal cortices (OFC) in the adult rat. We hypothesized that prenatal protein malnutrition would alter extracellular concentrations of cortical monoamines. The effects of an acute restraint stress were also assessed because PPM alters the brain’s response to stress. We used adult male, Long-Evans rats [10 prenatally malnourished (6% casein) and 10 prenatally well-nourished (25% casein)]. Samples were collected from the left and right hemispheres of the OFC every 20 min for 6 hr total and quantified using HPLC. After 2 hr of sampling, animals were exposed to a 40-min restraint stress. Extracellular levels of NE were significantly higher in PPM animals than in well-nourished controls across both hemispheres at all time-points. In contrast, baseline levels of 5HT and DA levels did not differ between nutritional groups. 5HT levels, but not NE or DA levels, were elevated compared to baseline levels in both nutritional groups and in both hemispheres during the first 20 min of stress exposure. These data highlight the impact of PPM on neuromodulatory systems and the profile of changes in response to acute stress. Additional studies are needed to determine how these basal and stress-related responses impact cognitive performance and whether these differences persist during cognitive testing.
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影响因子：
3.6
作者：
GALLER, JR;RAMSEY, FC;SALT, P
通讯作者：
SALT, P
影响因子：
2.2
作者：
Fournier, David A.;Skaug, Hans J.;Sibert, John
通讯作者：
Sibert, John
影响因子：
5.7
作者：
Bolla, KI;Eldreth, DA;Ernst, M
通讯作者：
Ernst, M
DOI：
10.1111/j.1469-7610.2010.02208.x
发表时间：
2010-07-01
影响因子：
7.6
作者：
Galler, J. R.;Bryce, C. P.;Harrison, R.
通讯作者：
Harrison, R.
DOI：
10.1016/j.pnpbp.2017.07.004
发表时间：
2018-07-13
影响因子：
5.6
作者：
Girotti M;Adler SM;Bulin SE;Fucich EA;Paredes D;Morilak DA
通讯作者：
Morilak DA