Transcriptome analysis of canola (Brassica napus) under salt stress at the germination stage.
Transcriptome analysis of canola (Brassica napus) under salt stress at the germination stage.
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DOI：
10.1371/journal.pone.0116217
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Long W;Zou X;Zhang X
油菜是世界上最重要的油料作物之一。但盐胁迫限制了其产量。在本研究中，使用数字基因表达（DGE）在H2O（对照）和NaCl处理B后0、3、12和24小时探索转录组谱。油菜根在萌发阶段。在标记定位到测序的芜菁基因组后，进行对照和处理之间的基因表达的比较。对盐胁迫过程中差异表达的基因进行了研究，共筛选出163个在盐胁迫过程中差异表达的基因。基因本体论和京都基因百科全书和基因组富集分析表明，部分基因参与脯氨酸代谢、肌醇代谢、碳水化合物代谢和氧化还原过程，可能在萌发期盐胁迫响应中发挥重要作用。因此，本研究提供了新的候选盐胁迫响应基因，这些基因可能在盐胁迫抗性分子途径中的新节点发挥作用。
Canola (Brassica napus) is one of the most important oil crops in the world. However, its yield has been constrained by salt stress. In this study, transcriptome profiles were explored using Digital Gene Expression (DGE) at 0, 3, 12 and 24 hours after H2O (control) and NaCl treatments on B. napus roots at the germination stage. Comparisons of gene-expression between the control and the treatment were conducted after tag-mapping to the sequenced Brassica rapa genome. The differentially expressed genes during the time course of salt stress were focused on, and 163 genes were identified to be differentially expressed at all the time points. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that some of the genes were involved in proline metabolism, inositol metabolism, carbohydrate metabolic processes and oxidation-reduction processes and may play vital roles in the salt-stress response at the germination stage. Thus, this study provides new candidate salt stress responding genes, which may function in novel putative nodes in the molecular pathways of salt stress resistance.
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