Differential cross-linking and radio-protective effects of genipin on mature bovine and human patella tendons.
Differential cross-linking and radio-protective effects of genipin on mature bovine and human patella tendons.
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DOI：
10.1007/s10561-012-9295-3
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发表时间：
2013-03
影响因子：
1.5
通讯作者：
Maher, Suzanne A.
中科院分区：
文献类型：
--
作者：
Ng, Kenneth W.;Wanivenhaus, Florian;Chen, Tony;Abrams, Valarian D.;Torzilli, Peter A.;Warren, Russell F.;Maher, Suzanne A.
关键词：
伽马辐照是一种经过验证的灭菌方法，但并未广泛用于前交叉韧带（ACL）重建的同种异体移植物（例如，由于辐射引起的机械强度下降，导致髌骨肌腱）。解决这一缺点将提高同种异体移植物的安全性和供应，以满足当前和未来的需求。假设京尼平诱导的胶原交联将增加髌骨肌腱组织的拉伸模量，使得5 MRad伽马辐照不会将组织机械强度降低至低于原始未处理值。优化的京尼平处理使牛肌腱的拉伸模量增加约2.4倍。照射后，京尼平处理的组织与天然组织没有显著差异，证明了该假设。优化的京尼平处理人肌腱的拉伸模量增加了约1.3倍。照射后，对照组和京尼平治疗组的组织均具有其天然肌腱模量的~50-60%，从而反驳了该假设。这些结果突出了京尼平交联对肌腱组织可能的年龄和物种依赖性影响。人类同种异体移植物的交联可能仅在较年轻的供体组织中有益。未来的研究是必要的，以更好地了解胶原交联的机制和应用，为临床使用。
Gamma irradiation is a proven sterilization method, but is not widely used on allografts for anterior cruciate ligament (ACL) reconstruction (e.g., patella tendon) due to radiation-induced decreases in mechanical strength. Addressing this drawback would improve the safety and supply of allografts to meet current and future demand. It was hypothesized that genipin-induced collagen cross-linking would increase the tensile modulus of patella tendon tissue such that 5 MRad gamma irradiation would not reduce the tissue mechanical strength below the original untreated values. Optimized genipin treatment increased the tensile modulus of bovine tendons by ~2.4-fold. After irradiation, genipin treated tissue did not significantly differ from native tissue, proving the hypothesis. Optimized genipin treatment of human tendons increased the tensile modulus by ~1.3-fold. After irradiation, both control and genipin-treated tissues possessed ~50–60% of their native tendon modulus, disproving the hypothesis. These results highlight possible age- and species-dependent effects of genipin cross-linking on tendon tissue. Cross-linking of human allografts may be beneficial only in younger donor tissues. Future research is warranted to better understand the mechanisms and applications of collagen cross-linking for clinical use.
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影响因子：
2.8
作者：
GIBBONS, MJ;BUTLER, DL;NOYES, FR
通讯作者：
NOYES, FR
DOI：
10.1002/jbm.820240905
发表时间：
1990-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
HUANGLEE, LLH;CHEUNG, DT;NIMNI, ME
通讯作者：
NIMNI, ME
影响因子：
0.1
作者：
Beasley, Leslie S.;Weiland, Daniel E.;West, Robin, V
通讯作者：
West, Robin, V
影响因子：
14
作者：
Chen, YS;Chang, JY;Liu, BS
通讯作者：
Liu, BS
影响因子：
3.3
作者：
Haraldsson, BT;Aagaard, P;Magnusson, SP
通讯作者：
Magnusson, SP