Analysis of S-nitrosothiols via copper cysteine (2C) and copper cysteine-carbon monoxide (3C) methods.
Analysis of S-nitrosothiols via copper cysteine (2C) and copper cysteine-carbon monoxide (3C) methods.
复制标题
DOI：
10.1016/j.ymeth.2012.10.008
复制
发表时间：
2013-08-01
期刊：
影响因子：
4.8
通讯作者：
Doctor, Allan
中科院分区：
文献类型：
--
作者：
Rogers, Stephen C.;Gibbons, Lindsey B.;Griffin, Sherraine;Doctor, Allan
This chapter summarizes the principles of RSNO measurement in the gas phase, utilizing ozone-based chemiluminescence and the copper cysteine (2C) ± carbon monoxide (3C) reagent. Although an indirect method for quantifying RSNOs, this assay represents one of the most robust methodologies available. It exploits the NO• detection sensitivity of ozone based chemiluminscence, which is within the range required to detect physiological concentrations of RSNO metabolites. Additionally, the specificity of the copper cysteine (2C and 3C) reagent for RSNOs negates the need for sample pretreatment, thereby minimizing the likelihood of sample contamination (false positive results), NO species inter-conversion, or the loss of certain highly labile RSNO species. Herein, we outline the principles of this methodology, summarizing key issues, potential pitfalls and corresponding solutions.
登录
查看更多内容
影响因子：
3.9
作者：
ARNELLE, DR;STAMLER, JS
通讯作者：
STAMLER, JS
影响因子：
--
作者：
CLYNE, MAA;WAYNE, RP;THRUSH, BA
通讯作者：
THRUSH, BA
DOI：
10.1039/p29960000481
发表时间：
1996-04-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
影响因子：
--
作者：
Dicks, AP;Swift, HR;Cox, BG
通讯作者：
Cox, BG
DOI：
10.1006/bbrc.1998.9688
发表时间：
1998-11-27
影响因子：
3.1
作者：
Fang, KZ;Ragsdale, NV;Gaston, B
通讯作者：
Gaston, B
影响因子：
4.8
作者：
Gow, AJ;Buerk, DG;Ischiropoulos, H
通讯作者：
Ischiropoulos, H