Shear stress induced by fluid flow produces improvements in tissue-engineered cartilage.
Shear stress induced by fluid flow produces improvements in tissue-engineered cartilage.
复制标题
DOI：
10.1088/1758-5090/aba412
复制
发表时间：
2020-08-10
期刊：
影响因子：
9
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Salinas EY;Aryaei A;Paschos N;Berson E;Kwon H;Hu JC;Athanasiou KA
组织工程学的目标是创造可植入的生物材料，用于修复和再生受损组织。体外组织工程通常基于静态培养，其限制了对营养物的获取并且缺乏机械信号传导。使用剪切应力是有争议的，因为在某些情况下，它可以导致细胞死亡，而在其他情况下，它促进组织再生。为了了解剪切应力如何起作用以及它如何用于改善新组织功能，进行了一系列研究。首先，设计了一种可调装置，以确定新组织形成的最佳剪切应力水平。然后，计算流体动力学建模显示，该装置在组织工程软骨（新软骨）上施加了跨越三个数量级的流体诱导剪切（FIS）应力。随后确定了FIS应力的有益窗口，导致体外新软骨的机械性能提高高达3.6倍。在体内，新软骨成熟的胶原蛋白含量向天然值的两倍证明。然后证明FIS应力向人源性新软骨的转化，产生类似的机械性能改善，例如拉伸模量增加168%。为了了解FIS应激的有益作用，进行了一项机制研究，揭示了由FIS应激激活的软骨细胞初级纤毛上的机械门控复合物。这一系列研究将FIS应力作为一种有意义的机械刺激策略置于竞技场中，用于产生坚固且可平移的新组织，并证明了将FIS应力纳入组织培养的容易性。
Tissue engineering aims to create implantable biomaterials for the repair and regeneration of damaged tissues. In vitro tissue engineering is generally based on static culture, which limits access to nutrients and lacks mechanical signaling. Using shear stress is controversial because in some cases it can lead to cell death while in others it promotes tissue regeneration. To understand how shear stress works and how it may be used to improve neotissue function, a series of studies were performed. First, a tunable device was designed to determine optimal levels of shear stress for neotissue formation. Then, computational fluid dynamics modeling showed the device applies fluid-induced shear (FIS) stress spanning three orders of magnitude on tissue-engineered cartilage (neocartilage). A beneficial window of FIS stress was subsequently identified, resulting in up to 3.6-fold improvements in mechanical properties of neocartilage in vitro. In vivo, neocartilage matured as evidenced by the doubling of collagen content toward native values. Translation of FIS stress to human derived neocartilage was then demonstrated, yielding analogous improvements in mechanical properties, such as 168% increase in tensile modulus. To gain an understanding of the beneficial roles of FIS stress, a mechanistic study was performed revealing a mechanically gated complex on the primary cilia of chondrocytes that is activated by FIS stress. This series of studies places FIS stress into the arena as a meaningful mechanical stimulation strategy for creating robust and translatable neotissues, and demonstrates the ease of incorporating FIS stress in tissue culture.
登录
查看更多内容
DOI：
10.1007/978-0-387-74911-2_22
发表时间：
2008-01-01
期刊：
OXYGEN TRANSPORT TO TISSUE XXIX
影响因子：
--
作者：
Berson, R. Eric;Purcell, Matthew R.;Sharp, M. Keith
通讯作者：
Sharp, M. Keith
影响因子：
3.8
作者：
Chakraborty, Amlan;Chakraborty, Sutirtha;Haribabu, Bodduluri
通讯作者：
Haribabu, Bodduluri
影响因子：
2.8
作者：
Han, Sang-Kuy;Wouters, Wim;Herzog, Walter
通讯作者：
Herzog, Walter
影响因子：
4.6
作者：
Hilton, MJ;Tu, XL;Long, FX
通讯作者：
Long, FX
影响因子：
4.8
作者：
Eyre, David R.;Weis, Mary Ann;Wu, Jiann-Jiu
通讯作者：
Wu, Jiann-Jiu