Inhibition of glycosylation of herpes simplex virus glycoproteins: identification of antigenic and immunogenic partially glycosylated glycopeptides on the cell surface membrane.
Inhibition of glycosylation of herpes simplex virus glycoproteins: identification of antigenic and immunogenic partially glycosylated glycopeptides on the cell surface membrane.
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单纯疱疹病毒糖蛋白糖基化的抑制：细胞表面膜上抗原性和免疫原性部分糖基化糖肽的鉴定。
DOI：
10.1016/0042-6822(83)90458-0
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Levine,M
中科院分区：
文献类型：
--
作者：
Glorioso,J;Szczesiul,MS;Marlin,SD;Levine,M
单纯疱疹病毒1型（HSV-1）科斯株感染的细胞表面膜含有三种主要糖蛋白，gC（表观Mr 129 k）、gB（表观Mr 120 k）和gD（表观Mr 58 k）。在糖基化抑制剂2-脱氧-d-葡萄糖存在下进行的感染导致成熟种类的损失，同时出现较低分子量多肽，其可能是完全加工的糖蛋白的部分糖基化形式。2-脱氧-D-葡萄糖抑制感染的放射性标记细胞质提取物的特异性免疫沉淀鉴定了部分糖基化的蛋白质，命名为DG 92、DG 88和DG 53，它们与相应的成熟形式gB、gC和gD抗原相关。细胞表面放射性碘标记，结合特异性免疫沉淀，发现DG 88和DG 53是2-脱氧-D-葡萄糖抑制感染中转运到细胞表面的主要物种。DG 92容易在细胞质中检测到，但不在质膜上。用科斯突变体synLD 70感染的细胞不合成糖蛋白gC。在糖基化抑制的synLD 70感染中，在细胞质或质膜中均未检测到DG 88，表明DG 88和gC之间存在遗传关系。针对糖蛋白gC、gB和gD的多克隆和单克隆抗体使感染细胞对补体介导的免疫细胞溶解敏感。在抑制剂存在下感染的细胞仅通过gC和gD特异性抗体对裂解敏感。糖基化抑制细胞对抗gB单克隆抗体免疫溶解不敏感。这些发现证实，糖基化缺陷形式的gC和gD，而不是gB到达细胞表面的抑制剂的存在下，和的糖基化的干扰素诱导的改变不会导致抗原性的完全丧失。在存在或不存在干扰素诱导的溶细胞和中和抗体的情况下，用感染的同基因3 T3细胞接种小鼠。溶细胞抗体的主要部分针对gC，但抗gC抗体似乎在病毒中和中起次要作用。虽然对照感染细胞诱导的血清含有gC、gB和gD的沉淀抗体，但接种抑制剂处理的感染细胞的小鼠的血清仅具有较弱的针对gB的免疫沉淀活性。总之，这些发现已经鉴定了主要HSV糖蛋白的部分糖基化形式，并且表明这些部分糖基化多肽中的一些转运至细胞表面不需要完全糖基化。此外，糖肽的完全糖基化对于维持抗原性或免疫原性不是必需的，这表明至少一些由针对成熟糖蛋白的抗体识别的决定簇不受2-脱氧-d-葡萄糖诱导的碳水化合物改变的影响。
The surface membranes of cells infected with herpes simplex virus type 1 (HSV-1), strain KOS, contain three principal glycoproteins, gC (apparentMr129k), gB (apparentMr120k), and gD (apparentMr58k). Infections carried out in the presence of the glycosylation inhibitor 2-deoxy-d-glucose result in the loss of the mature species with the concurrent appearance of lower-molecular-weight polypeptides which are presumably partially glycosylated forms of the fully processed glycoproteins. Specific immunoprecipitation of radiolabeled cytoplasmic extracts of 2-deoxy-d-glucose-inhibited infections identified partially glycosylated proteins designated DG92, DG88, and DG53, which are antigenically related to the corresponding mature forms gB, gC, and gD. Cell surface radioiodination, in combination with specific immunoprecipitation, revealed that DG88 and DG53 were the principal species transported to the cell surface in 2-deoxy-d-glucose-inhibited infections. DG92 was readily detected in the cytoplasm but not on the plasma membrane. Cells infected with the KOS mutant,synLD70, did not synthesize glycoprotein gC. In glycosylation-inhibitedsynLD70 infections, DG88 was not detected in either the cytoplasm or plasma membrane, demonstrating a genetic relationship between DG88 and gC. Polyclonal and monoclonal antibodies directed against the glycoproteins gC, gB, and gD sensitized infected cells to complement-mediated immune cytolysis. Cells infected in the presence of the inhibitor were sensitized to lysis only by antibody specific for gC and gD. The glycosylation-inhibited cells were insensitive to immunolysis by anti-gB monoclonal antibody. These findings confirm that the glycosylation-deficient forms of gC and gD, but not gB reach the cell surface in the presence of inhibitor and that the inhibitor-induced alterations in glycosylation do not cause a complete loss of antigenicity. Inoculation of mice with syngeneic 3T3 cells infected in the presence or absence of inhibitor-induced cytolytic and neutralizing antibody. A major portion of the cytolytic antibody was directed against gC, but anti-gC antibody appeared to play a minor role in virus neutralization. While the serum induced by the control infected cells contained precipitating antibodies for gC, gB, and gD, the serum derived from mice inoculated with inhibitor-treated infected cells had only weak immunoprecipitating activity against gB. Together, these findings have identified partially glycosylated forms of the major HSV glycoproteins and show that complete glycosylation is not required for transport of some of these partially glycosylated polypeptides to the cell surface. Moreover, complete glycosylation of the glycopeptides is not essential for maintenance of antigenicity or immunogenicity, indicating that at least some determinants recognized by antibodies directed against the mature glycoproteins are not affected by 2-deoxy-d-glucose-induced carbohydrate alterations.
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影响因子：
7.8
作者：
Tartakoff, A;Vassalli, P;Detraz, M
通讯作者：
Detraz, M
DOI：
10.1016/s0021-9258(17)38161-9
发表时间：
1978-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
I. Tabas;S. Schlesinger;S. Kornfeld
通讯作者：
I. Tabas;S. Schlesinger;S. Kornfeld
影响因子：
5.4
作者：
KNOWLES, RW;PERSON, S
通讯作者：
PERSON, S
1 型单纯疱疹病毒特异性糖蛋白异常的凝集素结合特性
影响因子：
5.4
作者：
S. Olofsson;S. Jeansson;E. Lycke
通讯作者：
E. Lycke
包被的囊泡分两个连续阶段将新合成的膜糖蛋白从内质网转运到质膜。
DOI：
10.1073/pnas.77.2.780
发表时间：
1980
影响因子：
11.1
作者：
J. Rothman;R. Fine
通讯作者：
R. Fine