Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.
Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.
复制标题
质谱法定量分析线粒体中的甘油磷脂
DOI:
10.1007/978-1-4939-6824-4_7
复制
发表时间:
2017
影响因子:
--
通讯作者:
Tatsuta
中科院分区:
文献类型:
--
作者:
Tatsuta
Lipids draw increasing attention of cell biologists because of the wide variety of functions beyond their role as building blocks of cellular membranes. Mitochondrial membranes possess characteristic lipid compositions that are intimately associated with mitochondrial architecture and activities. Therefore, quantitative assessment of lipids in isolated mitochondria is of importance for mitochondrial research. Here, I describe our workflow for quantitative analysis of glycerophospholipids in mitochondria with a focus on purification of pure mitochondrial fractions from yeast and cultured mammalian cells as well as improved settings for the analysis of cardiolipin by nano-electrospray ionization mass spectrometry.
影响因子:
13.8
作者:
Claypool, Steven M.;Koehler, Carla M.
通讯作者:
Koehler, Carla M.
DOI:
10.1007/978-1-62703-487-6_1
发表时间:
2013-01-01
期刊:
MEMBRANE BIOGENESIS: METHODS AND PROTOCOLS
影响因子:
--
作者:
Oezbalci, Cagakan;Sachsenheimer, Timo;Bruegger, Britta
通讯作者:
Bruegger, Britta
DOI:
10.1007/978-1-4939-1258-2_13
发表时间:
2014
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
作者:
Wang,Miao;Han,Xianlin
通讯作者:
Han,Xianlin