Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.

Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.
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质谱法定量分析线粒体中的甘油磷脂

DOI:
10.1007/978-1-4939-6824-4_7
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发表时间:
2017
影响因子:
--
通讯作者:
Tatsuta
Tatsuta
中科院分区:
--
文献类型:
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作者:
Tatsuta

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脂质引起细胞生物学家越来越多的关注,因为它们除了作为细胞膜的构建块之外还有各种各样的功能。线粒体膜具有与线粒体结构和活动密切相关的特征性脂质成分。因此,定量测定线粒体脂质含量对线粒体研究具有重要意义。在这里,我描述了我们的工作流程,定量分析的甘油磷脂在线粒体中的纯化纯线粒体组分从酵母和培养的哺乳动物细胞,以及改进的设置,通过纳米电喷雾电离质谱分析心磷脂的重点。
Lipids draw increasing attention of cell biologists because of the wide variety of functions beyond their role as building blocks of cellular membranes. Mitochondrial membranes possess characteristic lipid compositions that are intimately associated with mitochondrial architecture and activities. Therefore, quantitative assessment of lipids in isolated mitochondria is of importance for mitochondrial research. Here, I describe our workflow for quantitative analysis of glycerophospholipids in mitochondria with a focus on purification of pure mitochondrial fractions from yeast and cultured mammalian cells as well as improved settings for the analysis of cardiolipin by nano-electrospray ionization mass spectrometry.
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发表时间: 2012-01
影响因子: 13.8
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