Endophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype.
Endophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype.
复制标题
DOI：
10.1186/s12866-014-0332-1
复制
发表时间：
2014-12-24
期刊：
影响因子：
4.2
通讯作者：
Seldin L
中科院分区：
文献类型：
--
作者：
da Silva DA;Cotta SR;Vollú RE;Jurelevicius Dde A;Marques JM;Marriel IE;Seldin L
尽管转基因植物有很多好处，但关于这些植物对微生物群落的可能影响，文献中仍然没有足够的数据。因此，本研究旨在研究两种转基因玉米基因型（表达杀虫bt毒素的MON810型和表达杀虫bt毒素和抗除草剂的PAT[磷脂- n-乙酰基转移酶]的TC1507型）的遗传修饰对其内生微生物群落的影响，并与接近等基因的非转基因玉米（对照）的微生物群落进行比较。利用变性梯度凝胶电泳（DGGE）技术和克隆文库构建技术，分别对内生菌群落（细菌、古生菌和真菌）的结构和组成进行了分析。DGGE分析和细菌群落克隆文库显示，TC1507基因型与其他两个基因型略有差异。基因型TC1507的内生细菌群落多样性高于其他基因型。虽然有些细菌属存在于所有基因型中，如伯克霍尔德氏菌属、无色杆菌属和窄养单胞菌属，但有些细菌属为TC1507基因型所特有。此外，与Enterobacter相关的otu仅在TC1507克隆文库中占优势。玉米基因型TC1507的内生细菌群落与玉米基因型MON810及其近等基因亲本基因型（非bt或对照）不同。所研究的玉米基因型之间的差异可能与只存在于转基因基因型TC1507中的PAT蛋白编码基因的插入有关。
Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810 – expressing the insecticidal Bt-toxin and TC1507 – expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N-acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507.
登录
查看更多内容
影响因子：
4.2
作者：
Hardoim PR;Andreote FD;Reinhold-Hurek B;Sessitsch A;van Overbeek LS;van Elsas JD
通讯作者：
van Elsas JD
影响因子：
3
作者：
Gruber, Helga;Paul, Vijay;Mueller, Martin
通讯作者：
Mueller, Martin
影响因子：
3.7
作者：
Johnston-Monje D;Raizada MN
通讯作者：
Raizada MN
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD
影响因子：
3.6
作者：
Chelius, MK;Triplett, EW
通讯作者：
Triplett, EW