Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology.
Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology.
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DOI：
10.1371/journal.pone.0020396
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Raizada MN
中科院分区：
文献类型：
--
作者：
Johnston-Monje D;Raizada MN
内生菌是生活在植物体内的非病原微生物。我们问内生物种是否保存在农业上重要的植物属玉米，因为它成为驯化从其野生祖先（teosinte）到现代玉米（玉米），并从墨西哥到加拿大。采用培养、克隆和16 SrDNA末端限制性片段长度多态性（TRFLP）分析方法，对4种不同类型的大刍草和10个不同玉米品种的籽粒进行了内生细菌的筛选。TRFLP数据的主成分分析表明，种子内生菌群落组成的变化与植物宿主的发育有关。然而，有一个核心的内生菌微生物群，在玉米种子中跨越进化、人种学和生态学的界限而保守。野生祖先中的大多数种子内生菌今天仍然存在于驯化的玉米中，尽管对种子周围硬果壳的古老选择可能改变了内生菌的丰度。四个TRFLP信号，包括两个预测代表梭菌属和类芽孢杆菌属物种在所有玉米基因型中是保守的，而培养显示肠杆菌属、甲基杆菌属、泛菌属和假单胞菌属物种是广泛分布的，γ-变形菌属是流行的类别。培养了26个不同的属，并评价了它们刺激植物生长、在无氮培养基上生长、溶解磷酸盐、螯合铁、分泌RNA酶、拮抗病原体、分解代谢乙烯前体、产生生长素和乙偶姻/丁二醇的能力。在这些性状中，磷酸盐溶解和乙偶姻/丁二醇的产生是最常见的。从巨大的墨西哥地方品种Mixteco，与100%的身份伯克霍尔德氏菌phytofirmans，隔离显着促进芽马铃薯生物量。GFP标记和玉米茎注射证实了几种种子内生菌可以通过植物系统地传播。一个种子分离物，阿氏肠杆菌，能够退出根和殖民根际。在生态学、作物驯化、选择和迁移的背景下讨论了玉米-微生物关系中的保护和多样性。
Endophytes are non-pathogenic microbes living inside plants. We asked whether endophytic species were conserved in the agriculturally important plant genus Zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from Mexico to Canada. Kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and DNA fingerprinting using terminal restriction fragment length polymorphism (TRFLP) of 16S rDNA. Principle component analysis of TRFLP data showed that seed endophyte community composition varied in relation to plant host phylogeny. However, there was a core microbiota of endophytes that was conserved in Zea seeds across boundaries of evolution, ethnography and ecology. The majority of seed endophytes in the wild ancestor persist today in domesticated maize, though ancient selection against the hard fruitcase surrounding seeds may have altered the abundance of endophytes. Four TRFLP signals including two predicted to represent Clostridium and Paenibacillus species were conserved across all Zea genotypes, while culturing showed that Enterobacter, Methylobacteria, Pantoea and Pseudomonas species were widespread, with γ-proteobacteria being the prevalent class. Twenty-six different genera were cultured, and these were evaluated for their ability to stimulate plant growth, grow on nitrogen-free media, solubilize phosphate, sequester iron, secrete RNAse, antagonize pathogens, catabolize the precursor of ethylene, produce auxin and acetoin/butanediol. Of these traits, phosphate solubilization and production of acetoin/butanediol were the most commonly observed. An isolate from the giant Mexican landrace Mixteco, with 100% identity to Burkholderia phytofirmans, significantly promoted shoot potato biomass. GFP tagging and maize stem injection confirmed that several seed endophytes could spread systemically through the plant. One seed isolate, Enterobacter asburiae, was able to exit the root and colonize the rhizosphere. Conservation and diversity in Zea-microbe relationships are discussed in the context of ecology, crop domestication, selection and migration.
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影响因子：
4.1
作者：
Deepa, C. K.;Dastager, Syed G.;Pandey, Ashok
通讯作者：
Pandey, Ashok
影响因子：
6.9
作者：
Abanda-Nkpwatt, Daniel;Muesch, Martina;Schwab, Wilfried
通讯作者：
Schwab, Wilfried
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
DOI：
10.1084/jem.111.3.407
发表时间：
1960-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
DUBOS RJ;SCHAEDLER RW
通讯作者：
SCHAEDLER RW
影响因子：
2.8
作者：
Estrada, P;Mavingui, P;Caballero-Mellado, J
通讯作者：
Caballero-Mellado, J