Alterations of Fungal Microbiota in Patients With Cholecystectomy.
Alterations of Fungal Microbiota in Patients With Cholecystectomy.
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DOI：
10.3389/fmicb.2022.831947
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发表时间：
2022
影响因子：
5.2
通讯作者：
Liu, Yulan
中科院分区：
文献类型：
--
作者：
Xu, Jun;Ren, Xinhua;Liu, Yun;Zhang, Yuanyuan;Zhang, Yiwen;Chen, Guodong;Huang, Qing;Liu, Qing;Zhou, Jianhua;Liu, Yulan
越来越多的证据表明，胆囊切除术后（PC）患者发生胃肠道术后合并症（如结直肠癌）的风险较高。虽然以前的研究涉及真菌在结肠癌发生中的作用，但很少有报道关注PC患者的真菌特征。我们招募了104名受试者，包括52名PC患者和52名非PC对照（CON），收集粪便，通过内部转录间隔区（ITS）1 rDNA测序检测真菌成分。结果表明，假丝酵母（C.）glabrata和Aspergillus（A.）PC患者中未分配菌富集，白色念珠菌耗竭。此外，术后时间是影响真菌组成的主要因素。机器学习识别出C. glabrata、光叶草A. Unassigned和C.白色念珠菌是区分PC和CON的三个生物标志物。为了研究真菌在结肠癌发生中的作用，通过组织病理学研究将PC组的受试者分为两个亚组，即PC无（non-CA）和癌前病变或结直肠癌（preCA_CRC）患者。C.结果表明，不同状态的PC患者体内光滑脂肪酸逐渐蓄积。总之，我们发现胆囊切除术患者存在真菌菌群失调，术后时间是影响真菌组成的一个有效因素。C.光滑可能与胆囊切除术后癌变有关。
Increasing evidence suggests a high risk of gastrointestinal postoperative comorbidities (such as colorectal cancer) in patients with postcholecystectomy (PC). Although previous studies implicated the role of fungi in colon carcinogenesis, few reports focused on the fungal profile in patients with PC. We enrolled 104 subjects, including 52 patients with PC and 52 non-PC controls (CON), for fecal collection to detect the fungal composition by an internal transcribed spacer (ITS) 1 rDNA sequencing. Data showed that Candida (C.) glabrata and Aspergillus (A.) Unassigned were enriched, and Candida albicans was depleted in patients with PC. In addition, postoperative duration was the main factor to affect the fungal composition. Machine learning identified that C. glabrata, A. Unassigned, and C. albicans were three biomarkers to discriminate patients with PC from CON subjects. To investigate the fungal role in colon carcinogenesis, the subjects of the PC group were divided into two subgroups, namely, patients with PC without (non-CA) and with precancerous lesions or colorectal cancer (preCA_CRC), by histopathological studies. C. glabrata was found to be gradually accumulated in different statuses of patients with PC. In conclusion, we found fungal dysbiosis in patients with cholecystectomy, and the postoperative duration was a potent factor to influence the fungal composition. The accumulation of C. glabrata might be connected with carcinogenesis after cholecystectomy.
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