TOR and RPS6 transmit light signals to enhance protein translation in deetiolating Arabidopsis seedlings.
TOR and RPS6 transmit light signals to enhance protein translation in deetiolating Arabidopsis seedlings.
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DOI：
10.1073/pnas.1809526115
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发表时间：
2018-12-11
影响因子：
11.1
通讯作者：
Wu SH
中科院分区：
文献类型：
--
作者：
Chen GH;Liu MJ;Xiong Y;Sheen J;Wu SH
光增强拟南芥光形态发生发育过程中成千上万的mRNA的翻译效率，但其潜在的分子机制仍然是难以捉摸的。在这里，我们表明，光激活生长素-雷帕霉素靶（TOR）-核糖体蛋白S6（RPS 6）途径，以提高在黄化拟南芥翻译。我们发现组成型光形态发生蛋白1（COP 1）在黑暗生长的幼苗中抑制TOR活性。光感受器对远红光和蓝光的感知使COP 1失活，这导致生长素-TOR-RPS 6途径的去抑制和增强的从头蛋白质合成。我们的研究揭示了一个光触发的翻译调控信号通路。这种复杂的调节也起到确保幼苗在黑暗中具有严格的skotomorphogenic发展和及时切换到光形态发生发展的作用。脱黄化是植物幼苗进入营养生长阶段并具有光合活性的一个重要发育过程。光信号通过触发转录组和翻译组的大规模重编程来启动这一重要的发育过程。与丰富的转录调控知识相比，这种光引发的翻译增强的分子机制仍然不清楚。在这里，我们表明，光增强的翻译是由光感受器，结构性光形态发生1（COP 1），植物激素生长素，雷帕霉素的目标（TOR），和核糖体蛋白S6（RPS 6）组成的光感知和信号通路。在去黄化的拟南芥幼苗中，光感受器，包括光敏色素A和隐花色素，感知远红光和蓝光，以抑制负调节因子COP 1，这导致生长素途径的激活，从而使RPS 6的TOR依赖性磷酸化。拟南芥突变体的TOR，RPS 6A，或RPS 6 B缺陷表现出延迟子叶开放，一个特征的去黄化过程，以确保及时的营养发育的幼苗。本研究提供了一个机制的观点，光引发的翻译增强在黄化拟南芥。
Light enhances the translation efficiency of thousands of mRNAs during photomorphogenic development in Arabidopsis, but the underlying molecular mechanism remains elusive. Here we show that light activates the auxin-target of rapamycin (TOR)-ribosome protein S6 (RPS6) pathway to enhance translation in deetiolating Arabidopsis. We discovered that CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) represses TOR activity in dark-grown seedlings. The perception of far-red and blue light by photoreceptors inactivates COP1, which leads to the derepression of the auxin-TOR-RPS6 pathway and enhanced de novo protein synthesis. Our study revealed a light-triggered signaling pathway for translational regulation. This sophisticated regulation also functions to ensure that young seedlings have strict skotomorphogenic development in the dark and a timely switch to photomorphogenic development. Deetiolation is an essential developmental process transforming young plant seedlings into the vegetative phase with photosynthetic activities. Light signals initiate this important developmental process by triggering massive reprogramming of the transcriptome and translatome. Compared with the wealth of knowledge of transcriptional regulation, the molecular mechanism underlying this light-triggered translational enhancement remains unclear. Here we show that light-enhanced translation is orchestrated by a light perception and signaling pathway composed of photoreceptors, CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1), the phytohormone auxin, target of rapamycin (TOR), and ribosomal protein S6 (RPS6). In deetiolating Arabidopsis seedlings, photoreceptors, including phytochrome A and cryptochromes, perceive far-red and blue light to inactivate the negative regulator COP1, which leads to activation of the auxin pathway for TOR-dependent phosphorylation of RPS6. Arabidopsis mutants defective in TOR, RPS6A, or RPS6B exhibited delayed cotyledon opening, a characteristic of the deetiolating process to ensure timely vegetative development of a young seedling. This study provides a mechanistic view of light-triggered translational enhancement in deetiolating Arabidopsis.
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