Differential encoding in prefrontal cortex projection neuron classes across cognitive tasks.
Differential encoding in prefrontal cortex projection neuron classes across cognitive tasks.
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DOI：
10.1016/j.cell.2020.11.046
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发表时间：
2021-01-21
期刊：
影响因子：
64.5
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Lui JH;Nguyen ND;Grutzner SM;Darmanis S;Peixoto D;Wagner MJ;Allen WE;Kebschull JM;Richman EB;Ren J;Newsome WT;Quake SR;Luo L
单细胞转录组学已被广泛应用于哺乳动物大脑中的神经元分类，而系统神经科学在历史上分析了皮层神经元的编码特性，而不考虑细胞类型。在这里，我们研究如何特定的转录组类型的小鼠前额叶皮层（PFC）投射神经元与轴突投射和编码特性在多个认知任务。结果发现，除PFC→PAG（periaqueductal gray）外，大多数类型投射到多个靶点，大多数靶点接受多个类型的投射。通过比较自由活动小鼠中两种选择任务中分子同质PFC→PAG类型与两种异质类型的Ca 2+活性，我们发现所有检测的任务相关信号都定性地存在于所有检测的类别中。然而，PAG投射神经元最有力的编码线索任务中的选择，而对侧PFC投射神经元最有力的编码奖励上下文在一个uncue任务。因此，任务信号被冗余地组织起来，但在具有特定分子解剖特征的细胞之间存在明显的定量偏差。作为分子和系统神经科学观点的桥梁，Lui等人研究了小鼠前额皮质投射神经元的特定转录组类型如何与轴突投射和多种认知任务的编码特性相关。
Single-cell transcriptomics has been widely applied to classify neurons in the mammalian brain, while systems neuroscience has historically analyzed the encoding properties of cortical neurons without considering cell types. Here we examine how specific transcriptomic types of mouse prefrontal cortex (PFC) projection neurons relate to axonal projections and encoding properties across multiple cognitive tasks. We found that most types projected to multiple targets, and most targets received projections from multiple types, except PFC→PAG (periaqueductal gray). By comparing Ca2+ activity of the molecularly homogeneous PFC→PAG type against two heterogeneous classes in several two-alternative choice tasks in freely moving mice, we found that all task-related signals assayed were qualitatively present in all examined classes. However, PAG-projecting neurons most potently encoded choice in cued tasks, whereas contralateral PFC-projecting neurons most potently encoded reward context in an uncued task. Thus, task signals are organized redundantly, but with clear quantitative biases across cells of specific molecular-anatomical characteristics. Bridging molecular and systems neuroscience perspectives, Lui et al. examine how specific transcriptomic types of mouse prefrontal cortex projection neurons relate to axonal projections and encoding properties across multiple cognitive tasks
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
56.9
作者：
FUSTER, JM;ALEXANDER, GE
通讯作者：
ALEXANDER, GE
影响因子：
25
作者：
DeNardo, Laura A.;Liu, Cindy D.;Luo, Liqun
通讯作者：
Luo, Liqun
影响因子：
16.2
作者：
Euston DR;Gruber AJ;McNaughton BL
通讯作者：
McNaughton BL
影响因子：
16.2
作者：
Asaad, WF;Rainer, G;Miller, EK
通讯作者：
Miller, EK