Fish protein hydrolysate in diets of turbot affects muscle fibre morphometry, and the expression of muscle growth‐related genes
Fish protein hydrolysate in diets of turbot affects muscle fibre morphometry, and the expression of muscle growth‐related genes
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大菱鲆日粮中的鱼蛋白水解物影响肌纤维形态以及肌肉生长相关基因的表达
DOI：
10.1111/anu.13129
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发表时间：
2020-10
影响因子：
3.5
通讯作者：
Mengqing Liang
中科院分区：
文献类型：
--
作者：
Yuliang Wei;Benxiang Li;Houguo Xu;Mengqing Liang
本研究旨在研究饲料中鱼蛋白水解物（FPH）对大菱鲆生长性能、肌纤维形态和肌肉生长相关基因表达的影响。对照饲料（FPH0）添加0 g/kg FPH，配制4种试验饲料，分别以45 （FPH4.5）、120 （FPH12）、180 （FPH18）和300 (FPH30) g/kg的FPH替代鱼粉。饲料FPH12和FPH18的特定生长率（SGR）高于饲料FPH0 (p< 0.05)， SGR与饲料FPH水平呈二次回归关系（p= 0.001,R2= 0.677）。与对照组相比，FPH12组的横断面积（CSA）和肌节长度增加（p< 0.05），且CSA与饲粮FPH水平呈二次回归关系（p= 0.006,R2= 0.574），肌节长度与饲粮FPH水平呈二次回归关系（p= 0.018,R2= 0.788）。适当水平的FPH可下调肌肉生长抑制素2基因表达，上调增殖细胞核抗原基因表达，而饮食处理不影响肌生成调节因子的表达（p < 0.05）。综上所述，适量的FPH可能通过调节肌肉生长相关基因的表达以及肌肉的微结构和超微结构来促进肌肉生长。
The study was conducted to investigate the effects of fish protein hydrolysate (FPH) in diets for turbot on growth performance, muscle fibre morphometry, and the expression of muscle growth‐related genes. A control diet (FPH0) contained 0 g/kg FPH, and four experimental diets were formulated replacing fishmeal with FPH at levels of 45 (FPH4.5), 120 (FPH12), 180 (FPH18) and 300 (FPH30) g/kg. Fish fed the FPH12 and FPH18 diets had higher specific growth rate (SGR) than fish fed the FPH0 diet (p< .05), and a quadratic regression was found between SGR and dietary FPH level (p= .001,R2= .677). Cross‐section area (CSA) and the length of sarcomere in the FPH12 group increased compared with the control group (p< .05), and a quadratic regression was observed between CSA and dietary FPH level (p= .006,R2= .574) and between sarcomere length and dietary FPH level (p= .018,R2= .788). An appropriate level of FPH down‐regulated myostatin 2 gene expression and up‐regulated proliferating cell nuclear antigen gene expression, while the expression of myogenic regulatory factors was not affected by dietary treatments (p> .05). To conclude, an appropriate level of FPH may improve muscle growth by regulating the expression of muscle growth‐related genes, and muscle microstructure and ultrastructure.
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影响因子：
6.1
作者：
Hagen, Orjan;Solberg, Christel;Johnston, Ian A.
通讯作者：
Johnston, Ian A.
影响因子：
4.2
作者：
R. Lieber;J. Fridén
通讯作者：
R. Lieber;J. Fridén
DOI：
10.5604/20804075.1024710
发表时间：
2012-12
期刊：
Advances in Science and Technology Research Journal
影响因子：
--
作者：
D. Guzek;Krzysztof Głąbski;D. Głąbska;P. Plewa;R. Plewa;A. Wierzbicka
通讯作者：
D. Guzek;Krzysztof Głąbski;D. Głąbska;P. Plewa;R. Plewa;A. Wierzbicka
影响因子：
3.2
作者：
G. H. Ospina-Salazar;M. G. Ríos-Durán;E. M. Toledo-Cuevas;C. Martínez-Palacios
通讯作者：
G. H. Ospina-Salazar;M. G. Ríos-Durán;E. M. Toledo-Cuevas;C. Martínez-Palacios
影响因子：
3.5
作者：
J. Xia;A. Weaver;D. Gerrard;G. Yao
通讯作者：
J. Xia;A. Weaver;D. Gerrard;G. Yao