Manduca sexta gloverin binds microbial components and is active against bacteria and fungi.
Manduca sexta gloverin binds microbial components and is active against bacteria and fungi.
复制标题
DOI：
10.1016/j.dci.2012.06.012
复制
发表时间：
2012-10
影响因子：
2.9
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Xu, Xiao-Xia;Zhong, Xue;Yi, Hui-Yu;Yu, Xiao-Qiang
关键词：
Hyalophora gloveri gloverin是一种富含甘氨酸且热稳定的抗菌蛋白，主要对大肠杆菌具有抗菌活性。然而，甜菜夜蛾Gloverin对革兰氏阳性细菌是有活性的，但对E.杆菌本研究旨在研究野牡丹（Manduca sexta gloverin，MsGlv）基因的表达谱、结合能力及抑菌活性。MsGlv在初孵幼虫的几种组织中均有表达，且在中肠和睾丸中表达量较高。活性Spätzle-C108和大肠杆菌肽聚糖（PGs）可上调MsGlv mRNA的表达。大肠杆菌和金黄色葡萄球菌，并通过预先注射抗M.提示MsGlv的表达受Toll-Spätzle通路的调控。重组MsGlv与O-特异性抗原、脂多糖（LPS）外核糖、革兰氏阳性脂磷壁酸（LTA）和PG以及海带多糖结合，但不与E.大肠杆菌PG或甘露聚糖。MsGlv对蜡样芽孢杆菌、酿酒酵母和新型隐球菌有活性，但对大肠杆菌几乎无活性。coli和革兰氏阳性菌S.金黄色的我们的研究结果表明，手套花素对某些细菌和真菌具有活性。
Hyalophora gloveri gloverin is a glycine-rich and heat stable antimicrobial protein with activity mainly against Escherichia coli. However, Spodoptera exigua gloverin is active against a Gram-positive bacterium but inactive against E. coli. In this study, we investigated expression profile, binding ability and antimicrobial activity of Manduca sexta gloverin (MsGlv). MsGlv transcript was detected in several tissues of naïve larvae with higher levels in the midgut and testis. Expression of MsGlv mRNA in larvae was up-regulated by active Spätzle-C108 and peptidoglycans (PGs) of E. coli and Staphylococcus aureus, and the activation was blocked by pre-injection of antibody to M. sexta Toll, suggesting that MsGlv expression is regulated by the Toll-Spätzle pathway. Recombinant MsGlv bound to the O-specific antigen and outer core carbohydrate of lipopolysaccharide (LPS), Gram-positive lipoteichoic acid (LTA) and PG, and laminarin, but not to E. coli PG or mannan. MsGlv was active against Bacillus cereus, Saccharomyces cerevisiae and Cryptococcus neoformans, but was almost inactive against E. coli and S. aureus. Our results suggest that gloverins are active against some bacteria and fungi.
登录
查看更多内容
影响因子：
3.4
作者：
Moreno-Habel DA;Biglang-awa IM;Dulce A;Luu DD;Garcia P;Weers PM;Haas-Stapleton EJ
通讯作者：
Haas-Stapleton EJ
影响因子：
2.9
作者：
Rao, Xiang-Jun;Yu, Xiao-Qiang
通讯作者：
Yu, Xiao-Qiang
影响因子：
3.8
作者：
Rao, Xiang-Jun;Xu, Xiao-Xia;Yu, Xiao-Qiang
通讯作者：
Yu, Xiao-Qiang
影响因子：
--
作者：
Ganesan, Sandhya;Aggarwal, Kamna;Paquette, Nicholas;Silverman, Neal
通讯作者：
Silverman, Neal
DOI：
10.1111/j.1432-1033.1997.00614.x
发表时间：
1997-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Axen, A;Carlsson, A;Bennich, H
通讯作者：
Bennich, H