Complete genome sequence of Treponema paraluiscuniculi, strain Cuniculi A: the loss of infectivity to humans is associated with genome decay.
Complete genome sequence of Treponema paraluiscuniculi, strain Cuniculi A: the loss of infectivity to humans is associated with genome decay.
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DOI：
10.1371/journal.pone.0020415
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Weinstock GM
中科院分区：
文献类型：
--
作者：
Šmajs D;Zobaníková M;Strouhal M;Čejková D;Dugan-Rocha S;Pospíšilová P;Norris SJ;Albert T;Qin X;Hallsworth-Pepin K;Buhay C;Muzny DM;Chen L;Gibbs RA;Weinstock GM
副钩端螺旋体是引起兔性螺旋体病的病原体。虽然它的基因组结构与梅毒的病原体梅毒螺旋体亚种梅毒螺旋体等其他致病树种有非常密切的关系，但它对人类不具传染性。在这项研究中，通过几种高通量测序策略的组合，确定了副楔形密螺旋体A株的基因组序列。与梅毒螺旋体基因组的总体大小(1,133,390个碱基)、排列方式和基因含量非常相似，而副梅毒螺旋体基因组中含有的假基因和基因片段数量(51个)明显多于梅毒螺旋体基因组。除假基因外，副钩端螺旋体基因组中还发现了33个差异基因。一组受影响的基因(84个中的32个)编码了Nichols基因组中已知或预测功能的蛋白质。这些蛋白质包括毒力因子、基因调控因子以及DNA修复和重组成分。大多数(52个或61.9%)的假基因和分化基因具有未知的功能。我们的结果表明，副梅毒螺旋体是从一个类似梅毒螺旋体的祖先进化而来的，并在对人类失去感染力的过程中适应了一个专门的宿主相关生态位(兔子)。副梅毒螺旋体中灭活或改变的基因是在梅毒螺旋体亚种的感染性和致病机制中重要的毒力因子的候选基因。
Treponema paraluiscuniculi is the causative agent of rabbit venereal spirochetosis. It is not infectious to humans, although its genome structure is very closely related to other pathogenic Treponema species including Treponema pallidum subspecies pallidum, the etiological agent of syphilis. In this study, the genome sequence of Treponema paraluiscuniculi, strain Cuniculi A, was determined by a combination of several high-throughput sequencing strategies. Whereas the overall size (1,133,390 bp), arrangement, and gene content of the Cuniculi A genome closely resembled those of the T. pallidum genome, the T. paraluiscuniculi genome contained a markedly higher number of pseudogenes and gene fragments (51). In addition to pseudogenes, 33 divergent genes were also found in the T. paraluiscuniculi genome. A set of 32 (out of 84) affected genes encoded proteins of known or predicted function in the Nichols genome. These proteins included virulence factors, gene regulators and components of DNA repair and recombination. The majority (52 or 61.9%) of the Cuniculi A pseudogenes and divergent genes were of unknown function. Our results indicate that T. paraluiscuniculi has evolved from a T. pallidum-like ancestor and adapted to a specialized host-associated niche (rabbits) during loss of infectivity to humans. The genes that are inactivated or altered in T. paraluiscuniculi are candidates for virulence factors important in the infectivity and pathogenesis of T. pallidum subspecies.
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影响因子：
3.2
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