Histochemical analysis of lymphatic endothelial cells in the pancreas of non‐obese diabetic mice
Histochemical analysis of lymphatic endothelial cells in the pancreas of non‐obese diabetic mice
复制标题
非肥胖糖尿病小鼠胰腺淋巴内皮细胞的组织化学分析
作者：
Peng Qu;R. C. Ji;S. Kato
采用5′-核苷酸酶（5′-Nase）酶组织化学和次级淋巴组织趋化因子（SLC/CCL 21）免疫组织化学方法，研究了非肥胖糖尿病（NOD）小鼠胰岛发育与胰岛功能结构变化的关系。整个胰腺的小叶间淋巴管对5′-Nase呈阳性，并依赖于血管和胰管。小叶内的最初的arrhatics是罕见的，偶尔运行在附近的小岛。在非胰岛化阶段，5′-Nase反应产物均匀分布在淋巴管内皮细胞（LECs）表面，CCL 21弱表达。随着胰岛炎的发展，淋巴管上的5′-Nase活性略有降低。增加的血糖值似乎与内皮衬里增加的CCL 21表达一致，特别是在邻近浸润的胰岛和组织的LEC表面上。淋巴细胞和树突状细胞（DC）通常位于结缔组织中，围绕淋巴管壁沉积5′-Nase沉淀物。随着浸润的加重，淋巴细胞和DC在淋巴管内聚集并表达高水平的CCL 21。最重要的发现是许多DC粘附于淋巴管，通过薄而凹陷的内皮壁迁移。DCs（或淋巴细胞）与LECs粘附表面5′-Nase活性增强。后者的特点是开放的细胞间连接和明显的细胞质突起。这些结果表明，LECs与DC和淋巴细胞密切相互作用，并在NOD小鼠胰岛炎病理过程中DC和淋巴细胞通过淋巴管迁移中发挥关键作用。
We studied the relationship between insulitic development and function–structural changes of pancreatic lymphatics in non‐obese diabetic (NOD) mice using combined 5′‐nucleotidase (5′‐Nase) enzyme histochemical and secondary lymphoid tissue chemokine (SLC/CCL21) immunohistochemical methods. Interlobular lymphatic vessels were positive for 5′‐Nase throughout the pancreas, and dependent on both blood vessels and pancreatic ducts. Intralobular initial lymphatics were rare and occasionally ran in the neighbourhood of islets. During the non‐insulitic stage, the 5′‐Nase‐reactive product was evenly distributed on the surface of lymphatic endothelial cells (LECs) with weak expression of CCL21. The activity of 5′‐Nase on lymphatic vessels became slightly reduced as insulitis developed. The increasing blood glucose values appeared to be consistent with an increasing CCL21 expression by the endothelial lining, especially on the surface of LECs adjacent to the infiltrated islets and tissues. Lymphocytes and dendritic cells (DCs) were frequently located in the connective tissue, surrounding the lymphatic wall with deposition of 5′‐Nase precipitates. As the infiltration became severe, lymphocytes and DCs accumulated within lymphatic vessels and expressed high levels of CCL21. The most significant finding was that many DCs adhered to lymphatic vessels, transmigrating via the thin and indented endothelial walls. The activity of 5′‐Nase was increased on the adhesion surface between DCs (or lymphocytes) and LECs. The latter were characterized by open intercellular junctions and obvious cytoplasmic protrusions. These results suggest that LECs closely interact with DCs and lymphocytes, and play a key role in the migration of DCs and lymphocytes via lymphatic vessels during the pathological processes of insulitis in NOD mice.
影响因子：
6
作者：
Hjelmström, P;Fjell, J;Ruddle, NH
通讯作者：
Ruddle, NH
影响因子：
32.4
作者：
Luther, SA;Lopez, T;Cyster, JG
通讯作者：
Cyster, JG
DOI：
10.1073/pnas.95.1.258
发表时间：
1998-01-06
影响因子：
11.1
作者：
Gunn, MD;Tangemann, K;Williams, LT
通讯作者：
Williams, LT