Mechanism of action of peptide YY to inhibit gastric motility.
Mechanism of action of peptide YY to inhibit gastric motility.
复制标题
肽YY抑制胃蠕动的作用机制。
DOI：
10.1016/0016-5085(91)90257-l
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发表时间：
1991
期刊：
影响因子：
29.4
通讯作者：
Chung,OY
中科院分区：
文献类型：
--
作者：
Wiley,JW;Lu,YX;Chung,OY
肽YY抑制上消化道运动的途径在很大程度上是未知的，并促使这项调查。对从豚鼠胃中获得的离体肌条和切片进行肌张力和[3 H]乙酰胆碱释放研究。肽YY [0.1-1000 nmol/L;半数最大效应浓度（EC 50），6 nmol/L]引起纵向肌条的浓度依赖性舒张，不受六甲双铵影响，但被阿托品和河豚毒素阻断，表明肽抑制节后胆碱能神经传递。此外，肽YY（1 μmol/L）可使阿托品和河豚毒素阻断的电刺激肌肉收缩减少42% ± 6%，为肽抑制乙酰胆碱释放提供了额外的证据。接下来，检查肽YY对钾诱发的[3 H]乙酰胆碱释放的影响以及肽是否抑制乙酰胆碱的环腺苷一磷酸依赖性释放。YY肽（1 μmol/L）抑制KCl（35 mmol/L）诱发的[~ 3 H]乙酰胆碱释放58% ± 6%。肽YY的抑制作用不受已知介导突触前抑制的多巴胺-2、α-2和阿片受体拮抗剂的影响。此外，肽YY分别使毛喉素和霍乱毒素诱发的乙酰胆碱的半数最大释放减少45% ± 6%和42% ± 8%，表明肽可以抑制环磷酸腺苷依赖性的乙酰胆碱释放。肽YY的这种作用被百日咳毒素逆转，百日咳毒素阻止与腺苷酸环化酶偶联的抑制性鸟嘌呤核苷酸结合蛋白的激活。总之，肽YY抑制豚鼠胃中的基础和刺激胆碱能神经传递。此外，肽YY通过百日咳毒素敏感机制拮抗环腺苷一磷酸介导的乙酰胆碱释放。
The pathway by which peptide YY inhibits upper gastrointestinal motility is largely unknown and prompted this investigation. Muscle tension and [3H]acetylcholine release studies were performed on isolated muscle strips and slices obtained from the guinea pig stomach. Peptide YY [0.1–1000 nmol/L; concentration of half-maximal effect (EC50), 6 nmol/L] caused concentration-dependent relaxation of longitudinally oriented muscle strips that was unaffected by hexamethonium but was blocked by atropine and tetrodotoxin, suggesting that the peptide inhibited postganglionic cholinergic neurotransmission. In addition, peptide YY (1 μmol/L) reduced by 42% ± 6% electrically stimulated muscle contractions that were blocked by atropine and tetrodotoxin, providing additional evidence that the peptide inhibits release of acetylcholine. Next, the effect of peptide YY on potassium-evoked release of [3H]acetylcholine and whether the peptide inhibits cyclic adenosine monophosphate-dependent release of acetylcholine were examined. Peptide YY (1 μmol/L) inhibited KCl (35 mmol/L)-evoked release of [3H]acetylcholine by 58% ± 6%. The inhibitory action of peptide YY was unaffected by antagonists for dopamine-2, α-2, and opiate receptors that are known to mediate presynaptic inhibition. In addition, peptide YY reduced halfmaximal forskolin and cholera toxin-evoked release of acetylcholine by 45% ± 6% and 42% ± 8%, respectively, suggesting that the peptide can inhibit cyclic adenosine monophosphate-dependent release of acetylcholine. This effect of peptide YY was reversed by pertussis toxin which prevents activation of the inhibitory guanine nucleotide binding protein coupled to adenylate cyclase. In summary, peptide YY inhibited basal and stimulated cholinergic neurotransmission in the guinea pig stomach. In addition, peptide YY antagonized cyclic adenosine monophosphate-mediated release of acetylcholine through a pertussis toxin-sensitive mechanism.
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影响因子：
29.4
作者：
I. Taylor
通讯作者：
I. Taylor
肽YY：狗的代谢及其对胰腺分泌的影响。
影响因子：
29.4
作者：
Pappas,TN;Debas,HT;Taylor,IL
通讯作者：
Taylor,IL
肽 YY 通过抑制狗体内缩胆囊素的释放来抑制胰腺分泌。
影响因子：
29.4
作者：
Lluis,F;Gomez,G;Fujimura,M;GreeleyJr,GH;Thompson,JC
通讯作者：
Thompson,JC
影响因子：
13.9
作者：
S. Snyder
通讯作者：
S. Snyder
肽 YY 抑制狗胰腺分泌的肾上腺素能途径。
影响因子：
29.4
作者：
S. Konturek;J. Bilski;W. Pawlik;J. Tasler;W. Domschke
通讯作者：
W. Domschke