Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes.
Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes.
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DOI：
10.1007/s00418-020-01942-1
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发表时间：
2021-04
影响因子：
2.3
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Du A;Li L;Jiao Z;Zhu G;Peng T;Li H
钙反应反式激活因子(CREST)是一种在有丝分裂后神经元中高度表达的核蛋白，参与调节神经细胞的细胞周期、分化和树突发育。该基因在成年大鼠睾丸中已有表达，但其在睾丸中的蛋白表达和分布规律尚不清楚。在本研究中，我们检测了CREST在成年大鼠和人睾丸中的分布，以及在出生后发育的大鼠睾丸中的表达模式。免疫组织化学分析表明，在人和大鼠的成年睾丸中，CREST选择性地分布在成熟的支持细胞中，而不在生精细胞中。在生后发育的大鼠睾丸中，在生精细胞分化的初期，CREST不仅在支持细胞中表达，而且在性细胞和生精细胞中也有表达。支持细胞分化过程中CREST免疫反应持续增强，成年期达到高峰。然而，随着生精细胞的分化，CREST免疫染色强度急剧下降，在分化后阶段消失。此外，BRG1和p300这两个在体内广泛表达的CREST相互作用蛋白，被发现与CREST共存于包括支持细胞在内的生精上皮细胞中。CREST在发育过程中的独特表达模式提示，CREST可能在生精上皮细胞的分化过程中起着调节作用。成年期支持细胞特异性表达的CREST提示，CREST可能是成熟支持细胞的一种新的生物标志物。本文的在线版本(10.1007/s00418-020-01942-1)包含向授权用户提供的补充材料。
Calcium-responsive transactivator (CREST), a nuclear protein highly expressed in postmitotic neurons, is involved in the regulation of cell cycle, differentiation and dendritic development of neuronal cells. Its mRNA has been detected in the testis of adult rat, whilst its protein expression and distribution pattern in the testis remain to be elucidated. In this study, we examined the distribution of CREST in the adult testes of both rats and human as well as the expression pattern of CREST in the testes of postnatal developing rats. In the adult testes of both human and rats, immunohistochemical analysis revealed that CREST was selectively distributed in the mature Sertoli cells but not in the spermatogenic cells. In the testes of postnatal developmental rats, CREST was expressed not only in Sertoli cells but also in the gonocytes and spermatogenic cells at the initial stage of spermatogenic cell differentiation. CREST immunoreactivity continued to increase in Sertoli cells during differentiation, reaching its peak in adulthood. However, CREST immunostaining intensity dramatically decreased as the spermatogenic cells differentiate, disappearing in the post-differentiation stage. Furthermore, Brg1 and p300, two CREST-interacting proteins ubiquitously expressed in the body, are found to be colocalized with CREST in the spermatogenic epithelial cells including Sertoli cells. The unique expression pattern of CREST in developing testis suggests that CREST might play regulatory roles in the differentiation of spermatogenic epithelial cells. The Sertoli cell-specific expression of CREST in the adulthood hints that CREST might be a novel biomarker for the mature Sertoli cells. The online version of this article (10.1007/s00418-020-01942-1) contains supplementary material, which is available to authorized users.
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影响因子：
25
作者：
Chesi, Alessandra;Staahl, Brett T.;Jovicic, Ana;Couthouis, Julien;Fasolino, Maria;Raphael, Alya R.;Yamazaki, Tomohiro;Elias, Laura;Polak, Meraida;Kelly, Crystal;Williams, Kelly L.;Fifita, Jennifer A.;Maragakis, Nicholas J.;Nicholson, Garth A.;King, Oliver D.;Reed, Robin;Crabtree, Gerald R.;Blair, Ian P.;Glass, Jonathan D.;Gitler, Aaron D.
通讯作者：
Gitler, Aaron D.
影响因子：
16.2
作者：
Qiu Z;Ghosh A
通讯作者：
Ghosh A
影响因子：
4.6
作者：
Kim, Yuna;Fedoriw, Andrew M.;Magnuson, Terry
通讯作者：
Magnuson, Terry
DOI：
10.1073/pnas.0709854105
发表时间：
2008-06-17
影响因子：
11.1
作者：
Johnston, Daniel S.;Wright, William W.;Jelinsky, Scott A.
通讯作者：
Jelinsky, Scott A.
影响因子：
3.6
作者：
Rendon, A;Hewetson, A;Lee, VH
通讯作者：
Lee, VH