Pancreatic pericytes originate from the embryonic pancreatic mesenchyme.
Pancreatic pericytes originate from the embryonic pancreatic mesenchyme.
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DOI：
10.1016/j.ydbio.2019.01.020
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发表时间：
2019-05-01
影响因子：
2.7
通讯作者：
Landsman, Limor
中科院分区：
文献类型：
--
作者：
Harari, Neta;Sakhneny, Lina;Khalifa-Malka, Laura;Busch, Anke;Hertel, Klemens J.;Hebrok, Matthias;Landsman, Limor
关键词：
周细胞的胚胎起源是异质的，在器官之间和器官内。虽然体腔器官的周细胞被认为是从间皮（一种单层鳞状上皮）分化而来，但胰腺周细胞的胚胎起源还有待报道。在这里，我们表明，成人胰腺周细胞起源于胚胎胰腺间充质。我们的分析表明，成年小鼠胰腺的周细胞起源于表达转录因子Nkx3.2的细胞。在胚胎胰腺中，Nkx3.2表达细胞构成多层间充质，其包围胰腺上皮并支持其发育中的多个事件。因此，我们追踪了胰腺间充质的命运。我们的分析表明，胰腺间充质细胞获得各种周细胞的特性，包括基因表达，典型的形态，和内皮周围的位置，在胚胎发生。重要的是，我们发现绝大多数胰腺间充质细胞在胚胎第13.5天已经分化为周细胞，并在胰腺器官发生的后期阶段逐渐获得更成熟的周细胞表型。因此，我们的研究表明，胚胎胰腺间充质作为成人胰腺周细胞的主要来源。由于其他体腔器官的周细胞被认为是从间皮分化而来，我们的研究结果指出这些细胞在胰腺中的独特起源。因此，我们的研究提出了一个复杂的体腔器官周细胞的个体发育。
The embryonic origin of pericytes is heterogeneous, both between and within organs. While pericytes of coelomic organs were proposed to differentiate from the mesothelium, a single-layer squamous epithelium, the embryonic origin of pancreatic pericytes has yet to be reported. Here, we show that adult pancreatic pericytes originate from the embryonic pancreatic mesenchyme. Our analysis indicates that pericytes of the adult mouse pancreas originate from cells expressing the transcription factor Nkx3.2. In the embryonic pancreas, Nkx3.2-expressing cells constitute the multilayered mesenchyme, which surrounds the pancreatic epithelium and supports multiple events in its development. Thus, we traced the fate of the pancreatic mesenchyme. Our analysis reveals that pancreatic mesenchymal cells acquire various pericyte characteristics, including gene expression, typical morphology, and periendothelial location, during embryogenesis. Importantly, we show that the vast majority of pancreatic mesenchymal cells differentiate into pericytes already at embryonic day 13.5 and progressively acquires a more mature pericyte phenotype during later stages of pancreas organogenesis. Thus, our study indicates the embryonic pancreatic mesenchyme as the primary origin to adult pancreatic pericytes. As pericytes of other coelomic organs were suggested to differentiate from the mesothelium, our findings point to a distinct origin of these cells in the pancreas. Thus, our study proposes a complex ontogeny of pericytes of coelomic organs.
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影响因子：
29.4
作者：
Verzi MP;Stanfel MN;Moses KA;Kim BM;Zhang Y;Schwartz RJ;Shivdasani RA;Zimmer WE
通讯作者：
Zimmer WE
DOI：
10.1097/med.0000000000000174
发表时间：
2015-08
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Romer AI;Sussel L
通讯作者：
Sussel L
影响因子：
8.2
作者：
Tang, Shiue-Cheng;Chiu, Yu-Chen;Fu, Ya-Yuan
通讯作者：
Fu, Ya-Yuan
影响因子：
16.6
作者：
Byrnes LE;Wong DM;Subramaniam M;Meyer NP;Gilchrist CL;Knox SM;Tward AD;Ye CJ;Sneddon JB
通讯作者：
Sneddon JB
影响因子：
14.9
作者：
Dreszer TR;Karolchik D;Zweig AS;Hinrichs AS;Raney BJ;Kuhn RM;Meyer LR;Wong M;Sloan CA;Rosenbloom KR;Roe G;Rhead B;Pohl A;Malladi VS;Li CH;Learned K;Kirkup V;Hsu F;Harte RA;Guruvadoo L;Goldman M;Giardine BM;Fujita PA;Diekhans M;Cline MS;Clawson H;Barber GP;Haussler D;James Kent W
通讯作者：
James Kent W