Circadian and Sleep Metabolomics Across Species.
Circadian and Sleep Metabolomics Across Species.
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DOI：
10.1016/j.jmb.2020.04.027
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发表时间：
2020-05-29
影响因子：
5.6
通讯作者：
Weljie AM
中科院分区：
文献类型：
--
作者：
Malik DM;Paschos GK;Sehgal A;Weljie AM
在正常的昼夜节律功能下，代谢控制是跨组织和行为的时间上的协调，具有24小时的周期。然而，昼夜节律的紊乱会导致代谢平衡的负面后果，包括能量或氧化还原失衡。然而，由于包括睡眠不足在内的许多因素，昼夜节律紊乱在当今社会中变得越来越普遍。通过对昼夜生物学和睡眠的代谢组学分析，已经揭示了两者的代谢后果。具体地说，两个主要的分析平台，质谱学和核磁共振波谱已经被用来研究分子钟和睡眠对整体代谢节律性的影响。例如，人类研究已经证明了代谢节律在人类生物学中的普遍存在，以及睡眠中断的泛代谢组后果。然而，人类研究仅限于外周代谢读数，主要是通过微创程序。为了进一步的组织和生物体特异性的研究，基于跨物种的分子时钟和睡眠的保守性，已经研究了一些模型系统。在这里，我们总结了人类研究以及使用小鼠、果蝇和斑马鱼进行的代谢组学研究的关键发现。虽然信息量很大，但现有文献的一个局限性是缺乏对代谢物池中动态合成或分解代谢的解释。在这个意义上，未来的工作结合了同位素示踪剂、特定代谢物报告和单细胞代谢组学，可能会提供一种探索感兴趣途径中动态活性的方法。
Under normal circadian function, metabolic control is temporally coordinated across tissues and behaviors with a 24-hour period. However, circadian disruption results in negative consequences for metabolic homeostasis including energy or redox imbalances. Yet, circadian disruption has become increasingly prevalent within today’s society due to many factors including sleep loss. Metabolic consequences of both have been revealed by metabolomics analyses of circadian biology and sleep. Specifically, two primary analytical platforms, mass spectrometry and nuclear magnetic resonance spectroscopy have been used to study molecular clock and sleep influences on overall metabolic rhythmicity. For example, human studies have demonstrated the prevalence of metabolic rhythms in human biology, as well as pan-metabolome consequences of sleep disruption. However, human studies are limited to peripheral metabolic readouts primarily through minimally invasive procedures. For further tissue- and organism-specific investigations, a number of model systems have been studied, based upon the conserved nature of both the molecular clock and sleep across species. Here we summarize human studies as well as key findings from metabolomics studies using mice, Drosophila, and zebrafish. While informative, a limitation in existing literature is a lack of interpretation regarding dynamic synthesis or catabolism within metabolite pools. To this extent, future work incorporating isotope tracers, specific metabolite reporters, and single-cell metabolomics may provide a means of exploring dynamic activity in pathways of interest.
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影响因子：
2.9
作者：
Bell, Lauren N.;Kilkus, Jennifer M.;Penev, Plamen D.
通讯作者：
Penev, Plamen D.
影响因子：
4.6
作者：
Bourdon, Allen K.;Spano, Giovanna Maria;Cirelli, Chiara
通讯作者：
Cirelli, Chiara
影响因子：
11.3
作者：
Baker CL;Loros JJ;Dunlap JC
通讯作者：
Dunlap JC
影响因子：
16
作者：
Aviram, Rona;Manella, Gal;Asher, Gad
通讯作者：
Asher, Gad
昼夜节律的代谢和能量学的融合。
DOI：
10.1126/science.1195027
发表时间：
2010-12-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bass J;Takahashi JS
通讯作者：
Takahashi JS