Gelatin methacrylate microspheres for controlled growth factor release.
Gelatin methacrylate microspheres for controlled growth factor release.
复制标题
DOI：
10.1016/j.actbio.2014.11.028
复制
发表时间：
2015-02
影响因子：
9.7
通讯作者：
McDevitt, Todd C.
中科院分区：
文献类型：
--
作者：
Nguyen, Anh H.;McKinney, Jay;Miller, Tobias;Bongiorno, Tom;McDevitt, Todd C.
由于其简单的制备方法、固有的静电结合特性和蛋白水解降解性，明胶已被广泛用作组织工程和再生医学应用中各种生物分子的递送载体。与传统的化学交联方法（如使用戊二醛）相比，甲基丙烯酸酯改性明胶提供了一种更好地控制水凝胶交联程度的替代方法。在这里，我们研究了具有不同交联密度（15-90%）的甲基丙烯酸明胶（GMA）微粒的物理性质和生长因子输送。与GA MPs相比，甲基丙烯酸化程度越低的MPs弹性模量降低，网孔尺寸越大，甲基丙烯酸化程度越高，模量越大，网孔尺寸越小。正如预期的那样，观察到微粒交联密度与降解之间呈负相关，最低交联的GMA MPs降解速度最快，与GA MPs相当。有趣的是，低交联密度的GMA MPs可以比传统的GA交联MPs装载高达10倍的相对量的生长因子，尽管GA MPs的明胶含量更高。此外，降低GMA交联密度导致骨形态发生蛋白4 （BMP4）和碱性成纤维细胞生长因子（bFGF）释放更完全，并且在胶原酶处理下释放速度加快。这些研究表明，GMA MPs通过增强相对结合能力和允许蛋白水解降解可调性，为生长因子递送提供了更灵活的平台，从而为生长因子递送提供了更有效的控制释放系统。
Gelatin has been commonly used as a delivery vehicle for various biomolecules for tissue engineering and regenerative medicine applications due to its simple fabrication methods, inherent electrostatic binding properties, and proteolytic degradability. Compared to traditional chemical cross-linking methods, such as the use of glutaraldehyde (GA), methacrylate modification of gelatin offers an alternative method to better control the extent of hydrogel cross-linking. Here we examined the physical properties and growth factor delivery of gelatin methacrylate (GMA) microparticles formulated with a wide range of different cross-linking densities (15–90%). Less methacrylated MPs had decreased elastic moduli and larger mesh sizes compared to GA MPs, with increasing methacrylation correlating to greater moduli and smaller mesh sizes. As expected, an inverse correlation between microparticle cross-linking density and degradation was observed, with the lowest cross-linked GMA MPs degrading at the fastest rate, comparable to GA MPs. Interestingly, GMA MPs at lower cross-linking densities could be loaded with up to a 10-fold higher relative amount of growth factor over conventional GA cross-linked MPs, despite an order of magnitude greater gelatin content of GA MPs. Moreover, a reduced GMA cross-linking density resulted in more complete release of bone morphogenic protein 4 (BMP4) and basic fibroblast growth factor (bFGF) and accelerated release rate with collagenase treatment. These studies demonstrate that GMA MPs provide a more flexible platform for growth factor delivery by enhancing the relative binding capacity and permitting proteolytic degradation tunability, thereby offering a more potent controlled release system for growth factor delivery.
登录
查看更多内容
影响因子：
10.8
作者：
Holland, TA;Tabata, Y;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
3.4
作者：
Nichol JW;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
14
作者：
Carpenedo, Richard L.;Bratt-Leal, Andrs M.;Marklein, Ross A.;Seaman, Scott A.;Bowen, Nathan J.;McDonald, John F.;McDevitt, Todd C.
通讯作者：
McDevitt, Todd C.
影响因子：
4.6
作者：
Hu, Xiaohong;Ma, Lie;Gao, Changyou
通讯作者：
Gao, Changyou