Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro.
Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro.
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DOI：
10.1155/2014/576358
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发表时间：
2014
影响因子：
4.3
通讯作者：
Kuroda S
中科院分区：
文献类型：
--
作者：
Yamamoto M;Nakata H;Hao J;Chou J;Kasugai S;Kuroda S
脂肪组织来源的基质细胞，称为ASCs，在再生应用中发挥重要作用。它们类似于间充质干细胞，因为它们的取之不尽，一般分化潜力和可塑性，并显示出一系列细胞特异性和分化簇（CD）标记物特征类似于其他体干细胞。表型或分化的变化与CD标记物密切相关。我们研究的目的是展示不同的成骨分化特性的ASCs群体。从皮下脂肪组织中提取鼠源性ASC的原代细胞批次，并使用流式细胞术分选细胞的表面蛋白分子CD 90和CD 105的表达。在细胞培养后，分析针对CD 90和CD 105分选的每个细胞群的成骨潜能。结果表明，ASC表现出不同的群体，具有不同的成骨分化特征：未分选的ASC刺激成骨培养基中与骨髓基质细胞（BMSC）相当的矿化结节形成，BMP 2的病毒转染加速了CD 90和/或CD 105阳性ASC中矿化结节的形成，14天后观察到CD 105表达降低。未来的研究评估不同的免疫表型的ASCs应进行发展细胞为基础的组织工程。
Adipose tissue-derived stromal cells, termed ASCs, play an important role in regenerative applications. They resemble mesenchymal stem cells owing to their inexhaustibility, general differentiation potential, and plasticity and display a series of cell-specific and cluster-of-differentiation (CD) marker profiles similar to those of other somatic stem cells. Variations in phenotypes or differentiation are intimately associated with CD markers. The purpose of our study was to exhibit distinct populations of ASCs with differing characteristics for osteogenic differentiation. The primary cell batch of murine-derived ASCs was extracted from subcutaneous adipose tissue and the cells were sorted for the expression of the surface protein molecules CD90 and CD105 using flow cytometry. Each cell population sorted for CD90 and CD105 was analyzed for osteogenic potency after cell culture. The results suggested that ASCs exhibit distinct populations with differing characteristics for osteogenic differentiation: unsorted ASCs stimulated comparable mineralized nodule formation as bone marrow stromal cells (BMSCs) in osteogenic medium and viral transfection for BMP2 accelerated the formation of mineralized nodules in CD90 and/or CD105 positive ASCs with observation of decrease in CD105 expression after 14 days. Future studies assessing different immunophenotypes of ASCs should be undertaken to develop cell-based tissue engineering.
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DOI：
10.1016/j.bbrc.2006.04.089
发表时间：
2006-06-30
影响因子：
3.1
作者：
Cho, Seung-Woo;Kim, Inok;Kim, Byung-Soo
通讯作者：
Kim, Byung-Soo
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
37.8
作者：
Miranville, A;Heeschen, C;Bouloumié, A
通讯作者：
Bouloumié, A
影响因子：
4.8
作者：
Levi, Benjamin;Wan, Derrick C.;Longaker, Michael T.
通讯作者：
Longaker, Michael T.