Analysis of Small RNAs in Streptococcus mutans under Acid Stress-A New Insight for Caries Research.
Analysis of Small RNAs in Streptococcus mutans under Acid Stress-A New Insight for Caries Research.
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酸性胁迫下变形链球菌中的小 RNA 分析为龋齿研究提供新见解
DOI：
10.3390/ijms17091529
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发表时间：
2016-09-14
影响因子：
5.6
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Liu S;Tao Y;Yu L;Zhuang P;Zhi Q;Zhou Y;Lin H
变形链球菌（S.变形菌）是导致龋齿的主要临床病原体。其耐酸性已被确定为在酸性条件下存活和致龋的重要毒力因子。小RNA（sRNAs）被认为是毒力和胁迫适应的关键调节因子。在这里，我们首次构建了三个暴露于酸性条件下的小尺寸sRNA文库，然后使用qRT-PCR进行验证。在不同的酸胁迫条件（pH 7.5、6.5、5.5和4.5）下，在三个时间点（0.5、1和2 h）进一步评估预测为与酸耐受性生物信息学相关的两种sRNA和靶基因的水平。同时，细菌的生长特性和活力进行了评估。我们获得了1879个sRNA，其读段计数至少为100。110个sRNA与已报道的S.变种人通过qRT-PCR验证了18种sRNA中的10种。在每个时间点，随着酸从pH 7.5增加到4.5，细菌的存活率下降。除pH4.5外，其它pH下细菌均能随时间增殖。sRNA的水平从pH 7.5到5.5逐渐降低，并且在pH 4.5中略微增加;然而，靶mRNA的表达水平在酸性条件下比在pH 7.5中上调。这些结果表明，一些sRNA在酸胁迫条件下被特异性诱导，涉及酸适应，并为探索S.变种人
Streptococcus mutans (S. mutans) is the major clinical pathogen responsible for dental caries. Its acid tolerance has been identified as a significant virulence factor for its survival and cariogenicity in acidic conditions. Small RNAs (sRNAs) are recognized as key regulators of virulence and stress adaptation. Here, we constructed three libraries of sRNAs with small size exposed to acidic conditions for the first time, followed by verification using qRT-PCR. The levels of two sRNAs and target genes predicted to be bioinformatically related to acid tolerance were further evaluated under different acid stress conditions (pH 7.5, 6.5, 5.5, and 4.5) at three time points (0.5, 1, and 2 h). Meanwhile, bacterial growth characteristics and vitality were assessed. We obtained 1879 sRNAs with read counts of at least 100. One hundred and ten sRNAs were perfectly mapped to reported msRNAs in S. mutans. Ten out of 18 sRNAs were validated by qRT-PCR. The survival of bacteria declined as the acid was increased from pH 7.5 to 4.5 at each time point. The bacteria can proliferate under each pH except pH 4.5 with time. The levels of sRNAs gradually decreased from pH 7.5 to 5.5, and slightly increased in pH 4.5; however, the expression levels of target mRNAs were up-regulated in acidic conditions than in pH 7.5. These results indicate that some sRNAs are specially induced at acid stress conditions, involving acid adaptation, and provide a new insight into exploring the complex acid tolerance for S. mutans.
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影响因子：
14.9
作者：
Geissmann T;Chevalier C;Cros MJ;Boisset S;Fechter P;Noirot C;Schrenzel J;François P;Vandenesch F;Gaspin C;Romby P
通讯作者：
Romby P
影响因子：
2.1
作者：
Lee, Heon-Jin;Hong, Su-Hyung
通讯作者：
Hong, Su-Hyung
影响因子：
5.4
作者：
Caldelari, Isabelle;Chao, Yanjie;Vogel, Joerg
通讯作者：
Vogel, Joerg
影响因子：
4.5
作者：
Acebo, Paloma;Martin-Galiano, Antonio J.;Amblar, Monica
通讯作者：
Amblar, Monica
影响因子：
3.2
作者：
Krastel, Kirsten;Senadheera, Dilani B.;Cvitkovitch, Dennis G.
通讯作者：
Cvitkovitch, Dennis G.