Parsing glucose entry into the brain: novel findings obtained with enzyme-based glucose biosensors.
Parsing glucose entry into the brain: novel findings obtained with enzyme-based glucose biosensors.
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DOI：
10.1021/cn5002304
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发表时间：
2015-01-21
影响因子：
5
通讯作者：
Wakabayashi, Ken T.
中科院分区：
文献类型：
--
作者：
Kiyatkin, Eugene A.;Wakabayashi, Ken T.
关键词：
脑组织中的细胞外葡萄糖水平反映了其从动脉血的梯度依赖性进入与其用于细胞代谢之间的动态平衡。在这项工作中，我们提出了几套以前发表和未发表的数据，通过使用酶为基础的葡萄糖生物传感器加上恒电位高速安培法在自由活动的大鼠。首先，我们考虑基本的方法学问题有关的可靠性电化学测量的细胞外葡萄糖水平在生理相关条件下的大鼠。第二，我们目前的数据，在神经核（NAc）的显着的环境刺激诱导的葡萄糖反应，并讨论局部神经元激活和快速葡萄糖进入脑组织之间的关系。第三，通过提供静脉内和胃内葡萄糖递送引起的NAc葡萄糖变化的数据，我们讨论了葡萄糖血液浓度变化后葡萄糖进入细胞外结构域的其他机制。最后，通过显示葡萄糖饮用行为期间NAc葡萄糖波动的模式，我们讨论了“主动”和“被动”葡萄糖进入大脑之间的关系，其与行为相关的代谢活化的连接，以及这些变化在行为调节中可能的功能意义。这些数据为神经血管耦合的“神经元”假说提供了坚实的实验支持，该假说假定神经元活动在快速调节血管张力、局部血流以及葡萄糖和氧气进入脑组织以维持活跃的细胞代谢中起关键作用。
Extracellular levels of glucose in brain tissue reflect dynamic balance between its gradient-dependent entry from arterial blood and its use for cellular metabolism. In this work, we present several sets of previously published and unpublished data obtained by using enzyme-based glucose biosensors coupled with constant-potential high-speed amperometry in freely moving rats. First, we consider basic methodological issues related to the reliability of electrochemical measurements of extracellular glucose levels in rats under physiologically relevant conditions. Second, we present data on glucose responses induced in the nucleus accumbens (NAc) by salient environmental stimuli and discuss the relationships between local neuronal activation and rapid glucose entry into brain tissue. Third, by presenting data on changes in NAc glucose induced by intravenous and intragastric glucose delivery, we discuss other mechanisms of glucose entry into the extracellular domain following changes in glucose blood concentrations. Lastly, by showing the pattern of NAc glucose fluctuations during glucose-drinking behavior, we discuss the relationships between “active” and “passive” glucose entry to the brain, its connection to behavior-related metabolic activation, and the possible functional significance of these changes in behavioral regulation. These data provide solid experimental support for the “neuronal” hypothesis of neurovascular coupling, which postulates the critical role of neuronal activity in rapid regulation of vascular tone, local blood flow, and entry of glucose and oxygen to brain tissue to maintain active cellular metabolism.
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大脑温度稳态：生理波动和病理转移。
DOI：
10.2741/3608
发表时间：
2010-01-01
期刊：
Frontiers in bioscience (Landmark edition)
影响因子：
--
作者：
Kiyatkin EA
通讯作者：
Kiyatkin EA
影响因子：
5
作者：
Hershey, Neil D.;Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.
DOI：
10.1523/jneurosci.0334-09.2009
发表时间：
2009-05-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dunn-Meynell AA;Sanders NM;Compton D;Becker TC;Eiki J;Zhang BB;Levin BE
通讯作者：
Levin BE
影响因子：
2.5
作者：
BOUTELLE, MG;STANFORD, C;BARTLETT, PN
通讯作者：
BARTLETT, PN
影响因子：
2.9
作者：
Canal, CE;McNay, EC;Gold, PE
通讯作者：
Gold, PE