Expression of 5-HT Relates to Stem Cell Marker LGR5 in Patients with Gastritis and Gastric Cancer.
Expression of 5-HT Relates to Stem Cell Marker LGR5 in Patients with Gastritis and Gastric Cancer.
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胃炎和胃癌患者5-HT表达与干细胞标志物LGR5相关
DOI：
10.1007/s10620-022-07772-6
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发表时间：
2023-05
影响因子：
3.1
通讯作者：
Zhu, Yuanmin
中科院分区：
文献类型：
--
作者：
Niu, Qian;Li, Lin;Zhang, Caili;Qi, Changhai;He, Qiufeng;Zhu, Yuanmin
5-羟色胺（5-HT）和干细胞标志物G蛋白偶联受体5（LGR 5）与胃肠道炎症和肿瘤发生有关。但5-HT与LGR 5的关系尚不清楚。目的探讨5-HT和LGR 5在胃炎和胃癌患者胃黏膜中的表达及相关性。本研究共纳入41例胃癌患者和98例慢性胃炎患者。采用实时定量聚合酶链反应（qPCR）检测胃黏膜中TPH 1、LGR 5和β-catenin mRNA的表达。免疫组织化学染色检测胃粘膜5-HT阳性细胞和LGR 5阳性细胞。多重免疫荧光检测5-HT与嗜铬粒蛋白A（CgA）、5-HT受体4（5-HTR 4）和LGR 5的共定位。胃癌组5-HT和LGR 5的表达明显高于慢性胃炎组（p < 0.05）。在非萎缩性胃炎、肠上皮化生和胃癌患者中，5-HT和LGR 5的阳性率依次升高，分别为18.52%、35.56%和75.61%; 5-HT和LGR 5在胃炎和胃癌中的表达呈正相关（p < 0.05）。胃炎患者TPH 1 mRNA和LGR 5 mRNA的表达水平也呈正相关（r = 0.7377，p < 0.001）。此外，5-HT与CgA部分共定位，5-HTR 4与LGR 5共定位于胃粘膜。胃粘膜5-HT合成增加可能对LGR 5阳性胃上皮干细胞产生影响。
5-Hydroxytryptamine (5-HT) and stem cells marker G-protein-coupled receptor 5 (LGR5) are associate with gastrointestinal inflammation and tumorigenesis. But the relationship between 5-HT and LGR5 is unclear. To explore the expression and correlation of 5-HT and LGR5 in gastric mucosa of patients with gastritis and gastric cancer (GC). A total of 41 patients with GC and 98 patients with chronic gastritis were included in this study. The expression of TPH1 mRNA, LGR5 mRNA and β-catenin mRNA in gastric mucosa were explored by Real-time Quantitative polymerase chain reaction (qPCR). 5-HT-positive cells and LGR5-positive cells in gastric mucosa were detected by immunohistochemistry stains. The co-localization of 5-HT and chromogranin A (CgA), 5-HT receptor4 (5-HTR4) and LGR5 were detected by multiplex immunofluorescence. The expression of 5-HT and LGR5 in patients with GC was significantly higher than patients with chronic gastritis (p < 0.05). The positive rate of 5-HT and LGR5 increased sequentially in the patients with non-atrophic gastritis, intestinal metaplasia and GC, which were 18.52%, 35.56% and 75.61% for 5-HT, and 27.78%, 40.91% and 95.12% for LGR5, respectively. The expression of 5-HT and LGR5 was positively correlated in gastritis and GC patients (p < 0.05). Moreover, the expression level of TPH1 mRNA and LGR5 mRNA was also positively correlated in gastritis patients (r = 0.7377, p < 0.001). Besides, 5-HT was partially co-localized with CgA, and 5-HTR4 was co-localized with LGR5 in gastric mucosa. The increase of 5-HT synthesis in gastric mucosa may have an impact on LGR5-positive gastric epithelial stem cells.
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影响因子：
21.3
作者：
Leushacke, Marc;Tan, Si Hui;Barker, Nick
通讯作者：
Barker, Nick
肠干细胞标志物Lgr5在结直肠癌中的表达及其临床病理意义
影响因子：
7.5
作者：
He, Songbing;Zhou, Hao;Zhang, Yanyun
通讯作者：
Zhang, Yanyun
DOI：
10.1007/s00432-020-03314-7
发表时间：
2020-07-15
影响因子：
3.6
作者：
Gzil, Arkadiusz;Zarebska, Izabela;Szylberg, Lukasz
通讯作者：
Szylberg, Lukasz
影响因子：
29.4
作者：
Koo, Bon-Kyoung;Clevers, Hans
通讯作者：
Clevers, Hans
影响因子：
3
作者：
Guo, Fei;Yang, Tao;Wang, Yu
通讯作者：
Wang, Yu