Production of EPSPS and bar gene double-herbicide resistant castor (Ricinus communis L.)
Production of EPSPS and bar gene double-herbicide resistant castor (Ricinus communis L.)
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EPSPS和bar基因双除草剂抗性蓖麻的生产
DOI：
10.1080/13102818.2020.1804450
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发表时间：
2020-01
影响因子：
1.4
通讯作者：
Fenglan Huang
中科院分区：
文献类型：
--
作者：
Huibo Zhao;Yong Zhao;Rui Luo;Lifeng Yang;Guorui Li;Jianjun Di;Mu Peng;Lili Li;Qi Wen;Xiaotian Liang;Mingda Yin;Yanpeng Wen;Fenglan Huang
蓖麻（Ricinus communis L.）是世界十大油料作物之一。中国是第二大蓖麻豆生产国，但中国的蓖麻豆种植面积一直在下降。这种下降的一个主要原因是杂草的流行和缺乏抗除草剂蓖麻植物品种。本研究采用农杆菌介导的基因转移方法，将抗草甘膦基因EPSPS（5-烯醇pyruvylshikimate-3-phosphate synthase， 5-烯醇pyruvylshikimate-3-phosphate synthase， 5-烯醇pyruvylshikimate-3-phosphate）和抗草甘膦基因Bar （phosphinothricin N-acetyltransferase, phosphinothricin N-acetyltransferase）分别导入蓖麻2129（高茎优秀恢复系）和CSR 181（矮茎优秀恢复系）中。转基因植株EPSPS和Bar基因的表达显著增加，对草甘膦和草铵膦的抗性显著增强。蛋白质组学分析显示，转基因矮茎植株中20种蛋白的含量存在差异。其中9种蛋白质参与光合作用，并间接与Bar基因的作用机制有关。8种蛋白参与防御、修复、应激反应、氧化磷酸化和代谢途径，并与EPSPS基因的作用机制间接相关。这些结果为选育高抗除草剂蓖麻品种奠定了基础。
Abstract Castor (Ricinus communis L.) is one of the world's top ten oil crops. China is the second largest producer of castor beans, but China's castor bean acreage has been declining. A principal reason for this decline is the prevalence of weeds and the absence of herbicide resistant varieties of castor plants. In this study, we used Agrobacterium-mediated gene transfer to introduce the EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene, which confers resistance to glyphosate, and the Bar (phosphinothricin N-acetyltransferase) gene, encoding resistance to glufosinate, into castor 2129 (high stem excellent recovery line) and CSR 181 (dwarf stem excellent recovery line). The transgenic plants exhibited significantly increased expression of the EPSPS and Bar genes and significantly increased resistance to glyphosate and glufosinate. Proteomic analysis showed differences in the amounts of 20 proteins in the dwarf stem transgenic plants. Nine of these proteins are involved in photosynthesis and indirectly related to the mechanism of action of the Bar gene. Eight proteins are involved in defense, repair, stress response, oxidative phosphorylation, and metabolic pathways and are indirectly related to the mechanism of action of the EPSPS gene. These results provide a foundation for the preparation of high herbicide resistant castor varieties.
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影响因子：
13.8
作者：
Hummel AW;Chauhan RD;Cermak T;Mutka AM;Vijayaraghavan A;Boyher A;Starker CG;Bart R;Voytas DF;Taylor NJ
通讯作者：
Taylor NJ
影响因子：
3.7
作者：
Salas-Perez RA;Saski CA;Noorai RE;Srivastava SK;Lawton-Rauh AL;Nichols RL;Roma-Burgos N
通讯作者：
Roma-Burgos N
影响因子：
4.6
作者：
Fartyal D;Agarwal A;James D;Borphukan B;Ram B;Sheri V;Agrawal PK;Achary VMM;Reddy MK
通讯作者：
Reddy MK
影响因子：
4.1
作者：
Sammons, Robert Douglas;Gaines, Todd A.
通讯作者：
Gaines, Todd A.
影响因子：
3.7
作者：
Cao G;Liu Y;Zhang S;Yang X;Chen R;Zhang Y;Lu W;Liu Y;Wang J;Lin M;Wang G
通讯作者：
Wang G