Direct-write bioprinting three-dimensional biohybrid systems for future regenerative therapies.
Direct-write bioprinting three-dimensional biohybrid systems for future regenerative therapies.
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DOI：
10.1002/jbm.b.31831
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发表时间：
2011-07
影响因子：
3.4
通讯作者：
Hoying, James B.
中科院分区：
文献类型：
--
作者：
Chang, Carlos C.;Boland, Eugene D.;Williams, Stuart K.;Hoying, James B.
再生医学寻求用工程生物或生物杂交系统修复或替换功能失调的组织。目前的临床再生模型利用简单均匀的组织结构，由培养在生物相容性支架上的细胞形成。未来的再生疗法将需要制造含有多种细胞类型和细胞外基质的复杂三维结构。我们相信，生物打印技术将在设计和构建未来的工程组织中发挥关键作用，用于基于细胞的再生疗法。本文介绍了目前最先进的生物打印技术，重点是直写生物打印。我们描述了一些成功的生物打印复合生物杂交构建体的过程和设备的考虑。此外，我们还为心血管应用中常用的水凝胶系统（Pluronic F127）提供了基线直写打印参数。通过三种市售针头尺寸（20 ga、25 ga和30 ga）在机械和气动打印后测量直写分配线（粘度范围为30 mPa *s至大于600×106 mPa*s的凝胶）。包含微血管细胞和分离的微血管片段的示例图案也被生物打印成复合3D结构。细胞和血管碎片在掺入生物混合结构后保持活力并维持体外行为。生物制品的直写生物打印提供了一种独特的方法来设计和制造复杂的多组分3D结构以供实验使用。我们希望我们的设计见解和直写生物打印的基线参数描述将为同事们将这种3D制造方法纳入未来的再生疗法提供有用的基础。
Regenerative medicine seeks to repair or replace dysfunctional tissues with engineered biological or biohybrid systems. Current clinical regenerative models utilize simple uniform tissue constructs formed with cells cultured onto biocompatible scaffolds. Future regenerative therapies will require the fabrication of complex three-dimensional constructs containing multiple cell types and extracellular matrices. We believe bioprinting technologies will provide a key role in the design and construction of future engineered tissues for cell-based and regenerative therapies. This review describes the current state-of-the-art bioprinting technologies, focusing on direct-write bioprinting. We describe a number of process and device considerations for successful bioprinting of composite biohybrid constructs. In addition, we have provided baseline direct-write printing parameters for a hydrogel system (Pluronic F127) often used in cardiovascular applications. Direct-write dispensed lines (gels with viscosities ranging from 30 mPa*s to greater than 600×106 mPa*s) were measured following mechanical and pneumatic printing via three commercially available needle sizes (20ga, 25ga, and 30ga). Example patterns containing microvascular cells and isolated microvessel fragments were also bioprinted into composite 3D structures. Cells and vessel fragments remained viable and maintained in vitro behavior after incorporation into biohybrid structures. Direct-write bioprinting of biologicals provides a unique method to design and fabricate complex, multi-component 3D structures for experimental use. We hope our design insights and baseline parameter descriptions of direct-write bioprinting will provide a useful foundation for colleagues to incorporate this 3D fabrication method into future regenerative therapies.
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影响因子：
3.1
作者：
Boland, ED;Matthews, JA;Bowlin, GL
通讯作者：
Bowlin, GL
影响因子：
2.8
作者：
Cimetta, Elisa;Pizzato, Sara;Elvassore, Nicola
通讯作者：
Elvassore, Nicola
影响因子：
--
作者：
Cohen, Daniel L.;Malone, Evan;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
4.6
作者：
Dos Reis, Gabriel;Fenili, Fabio;Milani, Paolo
通讯作者：
Milani, Paolo
影响因子：
3.8
作者：
Du, Yanan;Ghodousi, Majid;Lo, Edward;Vidula, Mahesh K.;Emiroglu, Onur;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali