Intrinsic depot-specific differences in the secretome of adipose tissue, preadipocytes, and adipose tissue-derived microvascular endothelial cells.
Intrinsic depot-specific differences in the secretome of adipose tissue, preadipocytes, and adipose tissue-derived microvascular endothelial cells.
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作者：
Hocking SL;Wu LE;Guilhaus M;Chisholm DJ;James DE
内脏脂肪组织（VAT）比皮下脂肪组织（SAT）与胰岛素抵抗的关系更密切。我们对VAT和SAT的分泌组进行了定量分析，以确定导致VAT不良代谢后果的脂肪因子分泌差异。我们使用凝集素亲和层析，然后通过比较同位素标记的氨基酸掺入率来定量VAT和SAT外植体分泌物的相对差异。由于脂肪组织由多种细胞类型组成，这可能导致储存库特异性分泌差异，因此我们分离了前脂肪细胞和微血管内皮细胞（MVEC），并将它们的分泌组与整个脂肪组织的分泌组进行比较。虽然有没有离散的储蓄具体的差异，从整个脂肪组织，前脂肪细胞，或MVECS的分泌，增值税表现出整体水平较高的蛋白质分泌比SAT。与SAT外植体（59%对21%）、前脂肪细胞（68%对0%）和MVEC（62%对15%）相比，VAT外植体分泌的蛋白质丰度是前者的两倍。整个脂肪组织分泌组中的蛋白质数量大于其细胞成分的总和。最后，几乎50%的脂肪组织分泌组由在血管生成中起作用的因子组成。VAT比SAT具有更高的分泌能力，这种差异是其细胞成分的内在特征。鉴于脂肪组织分泌蛋白中血管生成因子的数量，我们认为VAT代表了一个更容易扩张的组织库。
Visceral adipose tissue (VAT) is more closely linked to insulin resistance than subcutaneous adipose tissue (SAT). We conducted a quantitative analysis of the secretomes of VAT and SAT to identify differences in adipokine secretion that account for the adverse metabolic consequences of VAT. We used lectin affinity chromatography followed by comparison of isotope-labeled amino acid incorporation rates to quantitate relative differences in the secretomes of VAT and SAT explants. Because adipose tissue is composed of multiple cell types, which may contribute to depot-specific differences in secretion, we isolated preadipocytes and microvascular endothelial cells (MVECs) and compared their secretomes to those from whole adipose tissue. Although there were no discrete depot-specific differences in the secretomes from whole adipose tissue, preadipocytes, or MVECS, VAT exhibited an overall higher level of protein secretion than SAT. More proteins were secreted in twofold greater abundance from VAT explants compared with SAT explants (59% versus 21%), preadipocytes (68% versus 0%), and MVECs (62% versus 15%). The number of proteins in the whole adipose tissue secretome was greater than the sum of its cellular constituents. Finally, almost 50% of the adipose tissue secretome was composed of factors with a role in angiogenesis. VAT has a higher secretory capacity than SAT, and this difference is an intrinsic feature of its cellular components. In view of the number of angiogenic factors in the adipose tissue secretome, we propose that VAT represents a more readily expandable tissue depot.
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影响因子：
29
作者：
Crowe, Seamus;Wu, Lindsay E.;Watt, Matthew J.
通讯作者：
Watt, Matthew J.
影响因子：
64.8
作者：
HE, GP;MUISE, A;RO, HS
通讯作者：
RO, HS
影响因子：
15.9
作者：
FRIED, SK;RUSSELL, CD;BROLIN, RE
通讯作者：
BROLIN, RE
影响因子：
15.9
作者：
CID, MC;GRANT, DS;KLEINMAN, HK
通讯作者：
KLEINMAN, HK
DOI：
10.1073/pnas.0601752103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Gesta, S;Blüher, M;Kahn, CR
通讯作者：
Kahn, CR