Comparative analysis of antibody responses to outer surface protein (Osp)A and OspC in dogs vaccinated with Lyme disease vaccines.
Comparative analysis of antibody responses to outer surface protein (Osp)A and OspC in dogs vaccinated with Lyme disease vaccines.
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DOI：
10.1016/j.tvjl.2021.105676
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Marconi RT
中科院分区：
文献类型：
--
作者：
Camire AC;Hatke AL;King VL;Millership J;Ritter DM;Sobell N;Weber A;Marconi RT
莱姆病（LD）是一种由蜱传播的人类和犬科动物最常见的疾病。美国，是由螺旋体伯氏疏螺旋体。亚单位和疫苗可用于预防LD犬。LD疫苗，其由两株B的细胞裂解物组成。burgdorferi，含有超过1000种不同的蛋白质和细胞成分。相比之下，亚单位疫苗在组成上被定义并且由外表面蛋白（Osp）A或OspA和OspC嵌合位组成。在这项研究中，我们比较评估了OspA和OspC诱导的抗体应答，这些抗体应答是通过接种北美市售的所有犬白蛋白和亚单位LD疫苗诱导的。对犬接种两剂系列的指定疫苗（间隔3周）：亚单位-AC、亚单位-A、菌苗-1和菌苗-2。通过ELISA测定针对OspA和OspC的抗体滴度，并通过免疫印迹评估每种疫苗引发识别不同OspC蛋白（称为OspC类型）的抗体的能力。虽然所有疫苗都引发类似的OspA抗体应答，但只有亚基-AC触发了对不同OspC蛋白的稳健且广泛交叉反应性的抗体应答。中提供的数据提供了关于LD犬疫苗制剂中亚单位和亚单位对关键蜱虫和哺乳动物相抗原的疫苗诱导抗体应答的新信息。
Lyme disease (LD), the most common tick-borne disease of canines and humans in N. America, is caused by the spirochete Borreliella burgdorferi. Subunit and bacterin vaccines are available for the prevention of LD in dogs. LD bacterin vaccines, which are comprised of cell lysates of two strains of B. burgdorferi, contain over 1000 different proteins and cellular constituents. In contrast, subunit vaccines are defined in composition and consist of either outer surface protein (Osp)A or OspA and an OspC chimeritope. In this study, we comparatively assessed antibody responses to OspA and OspC induced by vaccination with all canine bacterin and subunit LD vaccines that are commercially available in North America. Dogs were administered a two-dose series of the vaccine to which they were assigned (3 weeks apart): Subunit-AC, Subunit-A, Bacterin-1, and Bacterin-2. Antibody titers to OspA and OspC were determined by ELISA and the ability of each vaccine to elicit antibodies that recognize diverse OspC proteins (referred to as OspC types) assessed by immunoblot. While all of the vaccines elicited similar OspA antibody responses, only Subunit-AC triggered a robust and broadly cross-reactive antibody response to divergent OspC proteins. The data presented within provide new information regarding vaccination-induced antibody responses to key tick and mammalian phase antigens by both subunit and bacterin LD canine vaccine formulations.
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影响因子：
2.6
作者：
Grosenbaugh DA;De Luca K;Durand PY;Feilmeier B;DeWitt K;Sigoillot-Claude C;Sajous ML;Day MJ;David F
通讯作者：
David F
影响因子：
3.6
作者：
Iyer, Radha;Caimano, Melissa J.;Schwartz, Ira
通讯作者：
Schwartz, Ira
影响因子：
9.4
作者：
Lagal, V;Postic, D;Baranton, G
通讯作者：
Baranton, G
影响因子：
3.1
作者：
Bockenstedt, LK;Hodzic, E;Barthold, SW
通讯作者：
Barthold, SW
DOI：
10.1007/s10482-014-0164-x
发表时间：
2014-06-01
影响因子：
2.6
作者：
Adeolu, Mobolaji;Gupta, Radhey S.
通讯作者：
Gupta, Radhey S.