Study on the enzyme's 1,3-positional specificity during lipozyme TL-mediated biodiesel production
Study on the enzyme's 1,3-positional specificity during lipozyme TL-mediated biodiesel production
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脂酶TL介导的生物柴油生产过程中酶1,3位特异性的研究
DOI：
10.1039/c5ra09432a
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发表时间：
2015-07
期刊：
影响因子：
3.9
通讯作者：
Liu Dehua
中科院分区：
文献类型：
--
作者：
Li Renwang;Du Wei;Lu Dianlan;Dai Lingmei;Liu Dehua
The 1,3-positional specificity of lipases plays an important role in obtaining high yield of products especially in the methanolysis for biodiesel production. In this paper, the effect of solvent and water activity on the enzyme's 1,3-positional specificity during lipozyme TL-catalyzed methanolysis of triglyceride (TAG) for biodiesel production was explored. Then the effect of organic solvent and water activity on the methanolysis of monoglyceride (MAG) was further carried out from the aspect of a kinetics study. It was found that either in the methanolysis of TAG or MAG, the 1,3-positional specificity of lipozyme TL correlated well with the log P of organic solvents. With the increase of the log P of the organic solvent, the enzyme's 1,3-positional specificity decreased. Interestingly, in each group of solvents (ketones, alkanes and chlorinated hydrocarbon), the 1,3-positional specificity of the lipase improved with water activity increasing from 0.11 to 0.53, while it decreased with water activity further increasing from 0.53 to 0.97. Further exploration on the related mechanism with a molecular dynamics simulation revealed that the organic solvent influenced the dehydration state of the lipase, which might subsequently influence the lipase's 1,3-positional specificity.
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影响因子：
--
作者：
Sunil Sabbani;E. Hedenström
通讯作者：
Sunil Sabbani;E. Hedenström
影响因子：
11.4
作者：
Yan Wang;Hong Wu;Min-hua Zong
通讯作者：
Yan Wang;Hong Wu;Min-hua Zong
DOI：
10.1007/s11746-012-2146-3
发表时间：
2013
期刊：
Journal of the American Oil Chemists' Society
影响因子：
--
作者：
H. Ren;Yang Li;Wei Du;De‐hua Liu
通讯作者：
H. Ren;Yang Li;Wei Du;De‐hua Liu
影响因子：
4.1
作者：
Duan, Zhang-Qun;Du, Wei;Liu, De-Hua
通讯作者：
Liu, De-Hua
影响因子：
1.8
作者：
Broos, J
通讯作者：
Broos, J