Upper and lower respiratory tract microbiota in horses: bacterial communities associated with health and mild asthma (inflammatory airway disease) and effects of dexamethasone.
Upper and lower respiratory tract microbiota in horses: bacterial communities associated with health and mild asthma (inflammatory airway disease) and effects of dexamethasone.
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马匹上和下呼吸道菌群：与健康和轻度哮喘（炎症性气道疾病）和地塞米松的作用相关的细菌群落。
DOI：
10.1186/s12866-017-1092-5
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发表时间：
2017-08-23
期刊：
影响因子：
4.2
通讯作者：
Léguillette R
中科院分区：
文献类型：
--
作者：
Bond SL;Timsit E;Workentine M;Alexander T;Léguillette R
马呼吸道的微生物组成，以及由于轻度马哮喘（也称为炎症性气道疾病（IAD））的差异尚未报道。控制马体内IAD的主要治疗方法是皮质类固醇。目的是表征与呼吸健康和IAD相关的上呼吸道和下呼吸道微生物群，并利用高通量测序研究地塞米松对这些细菌群落的影响。马呼吸道微生物群以变形菌门（43.85%）、放线菌门（21.63%）、厚壁菌门（16.82%）和拟杆菌门（13.24%）4大门为主。50个属的相对丰度为0.0.1%，其中鞘氨单胞菌和泛藻属丰度最高。健康马的上呼吸道和下呼吸道微生物群不同，下呼吸道丰富度减少，2个OTUs丰富度不同。健康马与IAD马下呼吸道细菌群落存在差异；气管群落中OTUs的丰度随疾病状况不同而不同，在IAD马中链球菌增加。地塞米松治疗对健康马和IAD马的下呼吸道微生物群均有影响，8个OTU丰度增加（包括链球菌），1个OTU丰度减少。健康马和IAD马的下呼吸道微生物群存在差异。进一步研究链球菌在IAD中的作用是有必要的。地塞米松治疗影响了下呼吸道微生物群，这表明控制使用地塞米松治疗的IAD马的细菌过度生长可能是治疗策略的一部分。本文的在线版本（doi:10.1186/s12866-017-1092-5）包含补充材料，可供授权用户使用。
The microbial composition of the equine respiratory tract, and differences due to mild equine asthma (also called Inflammatory Airway Disease (IAD)) have not been reported. The primary treatment for control of IAD in horses are corticosteroids. The objectives were to characterize the upper and lower respiratory tract microbiota associated with respiratory health and IAD, and to investigate the effects of dexamethasone on these bacterial communities using high throughput sequencing. The respiratory microbiota of horses was dominated by four major phyla, Proteobacteria (43.85%), Actinobacteria (21.63%), Firmicutes (16.82%), and Bacteroidetes (13.24%). Fifty genera had a relative abundance > 0.1%, with Sphingomonas and Pantoea being the most abundant. The upper and lower respiratory tract microbiota differed in healthy horses, with a decrease in richness in the lower airways, and 2 OTUs that differed in abundance. There was a separation between bacterial communities in the lower respiratory tract of healthy and IAD horses; 6 OTUs in the tracheal community had different abundance with disease status, with Streptococcus being increased in IAD horses. Treatment with dexamethasone had an effect on the lower respiratory tract microbiota of both heathy and IAD horses, with 8 OTUs increasing in abundance (including Streptococcus) and 1 OTU decreasing. The lower respiratory tract microbiota differed between healthy and IAD horses. Further research on the role of Streptococcus in IAD is warranted. Dexamethasone treatment affected the lower respiratory tract microbiota, which suggests that control of bacterial overgrowth in IAD horses treated with dexamethasone could be part of the treatment strategy. The online version of this article (doi:10.1186/s12866-017-1092-5) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/j.1939-1676.1991.tb00939.x
发表时间：
1991-03-01
影响因子：
2.6
作者：
COHN, LA
通讯作者：
COHN, LA
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
影响因子：
2.2
作者：
Burrell, MH;Wood, JLN;Mumford, JA
通讯作者：
Mumford, JA
影响因子：
2.2
作者：
Christley, RM;Hodgson, DR;Hodgson, JL
通讯作者：
Hodgson, JL