The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolution.
The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolution.
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DOI：
10.1038/s41588-017-0036-1
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发表时间：
2018-03
期刊：
影响因子：
30.8
通讯作者：
Amemiya CT
中科院分区：
文献类型：
--
作者：
Smith JJ;Timoshevskaya N;Ye C;Holt C;Keinath MC;Parker HJ;Cook ME;Hess JE;Narum SR;Lamanna F;Kaessmann H;Timoshevskiy VA;Waterbury CKM;Saraceno C;Wiedemann LM;Robb SMC;Baker C;Eichler EE;Hockman D;Sauka-Spengler T;Yandell M;Krumlauf R;Elgar G;Amemiya CT
海七鳃鳗（Petromyzonmarinus）作为重建脊椎动物进化的比较模式。为了使更多的知情分析，我们开发了一种新的七鳃鳗种系基因组组装，它整合了几个互补的数据集。这种高度连续的（染色体规模）组装的分析表明，染色体和全基因组复制在祖先脊椎动物和七鳃鳗基因组的进化中发挥了重要作用，包括携带六个七鳃鳗HOX簇的染色体。该组件还包含数百个基因，这些基因在七鳃鳗的早期发育过程中从体细胞中可重复地消除。比较分析表明，这些基因的gnathostome（小鼠）同源物经常标记的Polycomb抑制复合物（PRCs）在胚胎干细胞中，提示重叠的体细胞DNA消除和抑制/二价状态，由早期胚胎PRCs调节的调控逻辑。这种新的组合将加强由七鳃鳗独特的生物学和进化/比较视角所告知的各种研究。
The sea lamprey (Petromyzon marinus) serves as a comparative model for reconstructing vertebrate evolution. To enable more informed analyses, we developed a new assembly of the lamprey germline genome that integrates several complementary datasets. Analysis of this highly contiguous (chromosome-scale) assembly reveals that both chromosomal and whole-genome duplications have played significant roles in the evolution of ancestral vertebrate and lamprey genomes, including chromosomes that carry the six lamprey HOX clusters. The assembly also contains several hundred genes that are reproducibly eliminated from somatic cells during early development in lamprey. Comparative analyses show that gnathostome (mouse) homologs of these genes are frequently marked by Polycomb Repressive Complexes (PRCs) in embryonic stem cells, suggesting overlaps in the regulatory logic of somatic DNA elimination and repressive/bivalent states that are regulated by early embryonic PRCs. This new assembly will enhance diverse studies that are informed by lampreys’ unique biology and evolutionary/comparative perspective.
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影响因子：
56.9
作者：
Duret, L;Chureau, C;Avner, P
通讯作者：
Avner, P
影响因子：
14.9
作者：
Bairoch, A;Apweiler, R
通讯作者：
Apweiler, R
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64.8
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影响因子：
3.9
作者：
Doolittle, Russell F.
通讯作者：
Doolittle, Russell F.
影响因子：
7
作者：
Cantarel, Brandi L.;Korf, Ian;Yandell, Mark
通讯作者：
Yandell, Mark