Use of the Analysis of the Volatile Faecal Metabolome in Screening for Colorectal Cancer.
Use of the Analysis of the Volatile Faecal Metabolome in Screening for Colorectal Cancer.
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DOI：
10.1371/journal.pone.0130301
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Turner C
中科院分区：
文献类型：
--
作者：
Batty CA;Cauchi M;Lourenço C;Hunter JO;Turner C
结直肠癌的诊断是一种侵入性和昂贵的结肠镜检查，通常在阳性筛查试验后进行。不幸的是，现有的筛查测试缺乏特异性和敏感性，因此进行了许多不必要的结肠镜检查。在这里，我们报告一个潜在的新的结直肠癌筛查试验的基础上分析挥发性有机化合物（VOCs）的粪便样品的顶部空间。粪便样本来自粪便潜血样本（FOBT）阳性的受试者。受试者随后进行结肠镜检查，将其分为低风险（非癌症）和高风险（结直肠癌）组。挥发性有机化合物进行了分析，选择离子流管质谱（SIFT-MS），然后使用单变量和多变量统计方法进行了分析。最有可能来自硫化氢、二甲基硫醚和二甲基二硫醚的离子在高风险受试者的样本中统计学显著高于低风险受试者。使用多变量方法的结果表明，该测试对FOBT阳性样本的正确分类率为75%，特异性为78%，灵敏度为72%，为FOBT提供了一种潜在的有效替代方法。
Diagnosis of colorectal cancer is an invasive and expensive colonoscopy, which is usually carried out after a positive screening test. Unfortunately, existing screening tests lack specificity and sensitivity, hence many unnecessary colonoscopies are performed. Here we report on a potential new screening test for colorectal cancer based on the analysis of volatile organic compounds (VOCs) in the headspace of faecal samples. Faecal samples were obtained from subjects who had a positive faecal occult blood sample (FOBT). Subjects subsequently had colonoscopies performed to classify them into low risk (non-cancer) and high risk (colorectal cancer) groups. Volatile organic compounds were analysed by selected ion flow tube mass spectrometry (SIFT-MS) and then data were analysed using both univariate and multivariate statistical methods. Ions most likely from hydrogen sulphide, dimethyl sulphide and dimethyl disulphide are statistically significantly higher in samples from high risk rather than low risk subjects. Results using multivariate methods show that the test gives a correct classification of 75% with 78% specificity and 72% sensitivity on FOBT positive samples, offering a potentially effective alternative to FOBT.
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影响因子：
37.3
作者：
Denkert C;Budczies J;Weichert W;Wohlgemuth G;Scholz M;Kind T;Niesporek S;Noske A;Buckendahl A;Dietel M;Fiehn O
通讯作者：
Fiehn O
影响因子：
2.4
作者：
Barker, M;Rayens, W
通讯作者：
Rayens, W
影响因子：
7.1
作者：
Birkett, A;Muir, J;ODea, K
通讯作者：
ODea, K
影响因子：
--
作者：
Jia, Wenjing;Whitehead, Rebekah N.;Cole, Jeffrey A.
通讯作者：
Cole, Jeffrey A.
影响因子：
3.8
作者：
Center, Melissa M.;Jemal, Ahmedin;Ward, Elizabeth
通讯作者：
Ward, Elizabeth