Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments.
Transcriptome Analysis of the Nematode Caenorhabditis elegans in Acidic Stress Environments.
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DOI：
10.3389/fphys.2020.01107
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发表时间：
2020
影响因子：
4
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Cong Y;Yang H;Zhang P;Xie Y;Cao X;Zhang L
现代工业化石燃料燃烧造成的海洋酸化和酸雨导致生物生存环境pH值下降，对许多生物体产生一系列负面影响。然而，动物对酸性pH应激反应的潜在机制在很大程度上是未知的。本研究以秀丽隐杆线虫为动物模型，探讨生物体对pH下降反应的调控机制。通过转录组分析发现了酸性胁迫环境下两条主要的胁迫响应途径。首先，当pH值从6.33下降到4.33时，蠕虫通过上调col、nas和dpy基因来响应pH压力，这些基因是角质层合成和结构完整性所必需的。第二，当pH从4.33继续降低时，通过细胞色素P450途径基因（cyp、gst、ugt和ABC转运蛋白）代谢外源物质在保护线虫免受可能由更酸性环境产生的有毒物质的影响方面发挥了主要作用。同时，角质层合成的减缓可能是由于其保护能力不足。此外，我们在线虫中发现的系统调节模式也可能适用于其他无脊椎动物和脊椎动物在变化的pH环境中生存。因此，我们的数据可能为进一步研究确定响应和适应酸性pH胁迫的主基因奠定基础，也可能为改善生态恢复结果的评估和监测提供新的解决方案，或通过基因组编辑产生新的基因型，用于在具有挑战性的环境中恢复，特别是在全球气候变化的酸性胁迫背景下。
Ocean acidification and acid rain, caused by modern industries’ fossil fuel burning, lead to a decrease in the living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals’ response to acidic pH stress are largely unknown. In this study, we used the nematode Caenorhabditis elegans as an animal model to explore the regulatory mechanisms of organisms’ response to pH decline. Two major stress-responsive pathways were found through transcriptome analysis in acidic stress environments. First, when the pH dropped from 6.33 to 4.33, the worms responded to the pH stress by upregulation of the col, nas, and dpy genes, which are required for cuticle synthesis and structure integrity. Second, when the pH continued to decrease from 4.33, the metabolism of xenobiotics by cytochrome P450 pathway genes (cyp, gst, ugt, and ABC transporters) played a major role in protecting the nematodes from the toxic substances probably produced by the more acidic environment. At the same time, the slowing down of cuticle synthesis might be due to its insufficient protective ability. Moreover, the systematic regulation pattern we found in nematodes might also be applied to other invertebrate and vertebrate animals to survive in the changing pH environments. Thus, our data might lay the foundation to identify the master gene(s) responding and adapting to acidic pH stress in further studies, and might also provide new solutions to improve assessment and monitoring of ecological restoration outcomes, or generate novel genotypes via genome editing for restoring in challenging environments especially in the context of acidic stress through global climate change.
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影响因子：
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