Effect of substrate stiffness on early mouse embryo development.
Effect of substrate stiffness on early mouse embryo development.
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DOI：
10.1371/journal.pone.0041717
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rinaudo P
中科院分区：
文献类型：
--
作者：
Kolahi KS;Donjacour A;Liu X;Lin W;Simbulan RK;Bloise E;Maltepe E;Rinaudo P
越来越清楚的是，细胞对其微环境的生物物理线索非常敏感，并且这些线索在影响其行为方面发挥着重要作用。在这项研究中，我们研究了早期植入前胚胎是否对机械信号（即培养环境的弹性）敏感。为了测试这一点，我们开发了一种新的胚胎培养系统，可以精确定义胚胎环境的机械特性。当代胚胎培养的标准环境是聚苯乙烯培养皿（PD），其硬度（1 GPa）比子宫上皮（1 kPa）大六个数量级。为了更接近体内子宫环境的机械方面，我们使用聚二甲基硅氧烷 (PDMS) 或制造 3D I 型胶原凝胶（1 kPa 硬度，Col-1k 组）。将替代基质上的小鼠胚胎发育与培养皿上的小鼠胚胎发育进行比较；观察发育百分比、孵化频率和细胞数量。我们的结果表明胚胎对其培养的机械环境敏感。在 Col-1k 上培养的胚胎显示出发育为 2 细胞（68±15% vs. 59±18%）、囊胚（64±9.1% vs. 50±18%）和孵化囊胚阶段（54±25% vs. 21±16%）的频率显着更高，并且滋养外胚层细胞数量增加（TE，65±13 vs. TE）。 49±12 个细胞）与 PD 中培养的对照胚胎相比（平均值±标准差；p<.01）。将在 Col-1k 和 PD 上培养的胚胎转移到受体雌性体内并在胚胎第 12.5 天进行观察。两组胎儿数量相同，但 Col-1k 胎儿的胎盘比对照组大，表明植入前环境的持续影响。总之，植入前微环境的特征影响着床前后的生长。
It is becoming increasingly clear that cells are remarkably sensitive to the biophysical cues of their microenvironment and that these cues play a significant role in influencing their behaviors. In this study, we investigated whether the early pre-implantation embryo is sensitive to mechanical cues, i.e. the elasticity of the culture environment. To test this, we have developed a new embryo culture system where the mechanical properties of the embryonic environment can be precisely defined. The contemporary standard environment for embryo culture is the polystyrene petri dish (PD), which has a stiffness (1 GPa) that is six orders of magnitude greater than the uterine epithelium (1 kPa). To approximate more closely the mechanical aspects of the in vivo uterine environment we used polydimethyl-siloxane (PDMS) or fabricated 3D type I collagen gels (1 kPa stiffness, Col-1k group). Mouse embryo development on alternate substrates was compared to that seen on the petri dish; percent development, hatching frequency, and cell number were observed. Our results indicated that embryos are sensitive to the mechanical environment on which they are cultured. Embryos cultured on Col-1k showed a significantly greater frequency of development to 2-cell (68±15% vs. 59±18%), blastocyst (64±9.1% vs. 50±18%) and hatching blastocyst stages (54±25% vs. 21±16%) and an increase in the number of trophectodermal cell (TE,65±13 vs. 49±12 cells) compared to control embryos cultured in PD (mean±S.D.; p<.01). Embryos cultured on Col-1k and PD were transferred to recipient females and observed on embryonic day 12.5. Both groups had the same number of fetuses, however the placentas of the Col-1k fetuses were larger than controls, suggesting a continued effect of the preimplantation environment. In summary, characteristics of the preimplantation microenvironment affect pre- and post-implantation growth.
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DOI：
10.1038/nrm3112
发表时间：
2011-05
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
5.5
作者：
Fowden, AL;Ward, JW;Constancia, M
通讯作者：
Constancia, M
影响因子：
4.3
作者：
Murayama, Yoshinobu;Mizuno, Jinji;Omata, Sadao
通讯作者：
Omata, Sadao
影响因子：
0.7
作者：
Crawford-Young, SJ
通讯作者：
Crawford-Young, SJ