Bioaerosol sampling: sampling mechanisms, bioefficiency and field studies.

Bioaerosol sampling: sampling mechanisms, bioefficiency and field studies.
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DOI:
10.1016/j.jhin.2016.03.017
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发表时间:
2016-07
期刊:
The Journal of hospital infection
影响因子:
--
通讯作者:
Williams C
Williams C
中科院分区:
其他
文献类型:
--
作者:
Haig CW;Mackay WG;Walker JT;Williams C

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世纪以来,对医疗环境中病原体可疑空气传播的调查一直是研究人员面临的挑战。由于每种病原体对环境条件和所经历的机械应力都表现出独特的反应,因此取样装置的选择并不明显。我们的目的是审查生物气溶胶采样,采样设备和方法。进行了全面的文献检索,使用电子数据库检索生物气溶胶采样的英文论文。该综述介绍了流行的生物气溶胶采样装置,如撞击器,旋风分离器,撞击器和过滤器的机制,解释了它们的优点和缺点,以及微生物生物效率的后果。许多成功的研究都指出了生物气溶胶采样的最佳实践,从使用小型个人采样器监测工人的病原体暴露到大型静态采样器在各种医疗环境中收集空气中的微生物。最重要的是要求研究应首先在实验室条件下确定所选取样器和所调查病原体的生物效率。在这些基础上,在复杂的实地对生物气溶胶材料进行采样,更有把握成功捕获低浓度的空气传播病原体。从实验室到现场使用,这篇综述使研究者能够对生物气溶胶采样器的选择及其应用做出明智的决定。
Investigations into the suspected airborne transmission of pathogens in healthcare environments have posed a challenge to researchers for more than a century. With each pathogen demonstrating a unique response to environmental conditions and the mechanical stresses it experiences, the choice of sampling device is not obvious. Our aim was to review bioaerosol sampling, sampling equipment, and methodology. A comprehensive literature search was performed, using electronic databases to retrieve English language papers on bioaerosol sampling. The review describes the mechanisms of popular bioaerosol sampling devices such as impingers, cyclones, impactors, and filters, explaining both their strengths and weaknesses, and the consequences for microbial bioefficiency. Numerous successful studies are described that point to best practice in bioaerosol sampling, from the use of small personal samplers to monitor workers' pathogen exposure through to large static samplers collecting airborne microbes in various healthcare settings. Of primary importance is the requirement that studies should commence by determining the bioefficiency of the chosen sampler and the pathogen under investigation within laboratory conditions. From such foundations, sampling for bioaerosol material in the complexity of the field holds greater certainty of successful capture of low-concentration airborne pathogens. From the laboratory to use in the field, this review enables the investigator to make informed decisions about the choice of bioaerosol sampler and its application.
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