Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats.
Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats.
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DOI：
10.1186/1743-7075-5-31
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发表时间：
2008-11-10
影响因子：
4.5
通讯作者：
Christensen, Merrill J.
中科院分区：
文献类型：
--
作者：
Tolman, Jessica R.;Lephart, Edwin D.;Setchell, Kenneth D. R.;Eggett, Dennis L.;Christensen, Merrill J.
饮食中摄入大量的硒或维生素E可降低前列腺癌的风险因素。我们测试了这两种饮食成分的联合补充是否比单独补充每种成分更能降低大鼠前列腺癌的风险因素。采用2 × 2析因设计，雄性Noble大鼠幼仔从受孕开始就暴露于含有足够（0.33-0.45 mg/kg饮食）或高（3.33-3.45 mg/kg）浓度硒（以Se-甲基硒代半胱氨酸形式）和低（10 mg/kg）或高（600 mg/kg）水平的异黄酮的饮食。幼仔食用各自的饲料，直至35、100或200天处死。在青春期后开始暴露于饲料的雄性Noble大鼠育种者在第336天处死。大鼠每两周称重一次。在处死时采集血液，解剖并称重体脂和前列腺。血清瘦素、IGF-1和睾酮水平用ELISA试剂盒测定。GC/MS法测定血清中硒的含量。肝硒依赖性谷胱甘肽过氧化物酶1的活性作为硒状态的指标。在喂食高与低维生素C饲料的幼仔中，35日龄时血清维生素C浓度几乎高出100倍（1187.1与14.4 ng/mL，平均值± SD），并且在100和200日龄时以及育种者中保持如此。在幼犬或育种犬中，肝脏谷胱甘肽过氧化物酶活性没有饮食差异。从35天开始，高剂量的维生素E摄入量显著（p = 0.001-0.047）降低了大鼠幼崽的体重，但在育种者中没有。高胆甾酮同样显著降低了幼仔的体脂和瘦素，而对繁殖者的影响不太明显，但仍然很显著。高摄入量的硒和维生素E酮分别降低血清IGF-1的幼崽在100和200天，但不是在育种。没有观察到一致的饮食对血清睾酮或前列腺相对重量的影响。在幼鼠中，高胆固醇和高硒的组合产生了最低的体重增加，最低的血清瘦素，和最低的血清IGF-1浓度的所有四种饮食。联合摄入高硒和高硒酮可能比单独摄入任何一种化合物获得更大的化学预防效果。补充的时机可能决定其效果的重要性。
High dietary intake of selenium or isoflavones reduces risk factors for prostate cancer. We tested whether combined supplementation of these two dietary components would reduce prostate cancer risk factors in rats more than supplementation of each component individually. Male Noble rat pups were exposed from conception to diets containing an adequate (0.33–0.45 mg/kg diet) or high (3.33–3.45 mg/kg) concentration of selenium as Se-methylselenocysteine and a low (10 mg/kg) or high (600 mg/kg) level of isoflavones in a 2 × 2 factorial design. Pups consumed their respective diets until sacrifice at 35, 100, or 200 days. Male Noble rat breeders, whose exposure to the diets began after puberty, were sacrificed at 336 days. Rats were weighed biweekly. Blood was collected at the time of sacrifice and body fat and prostates were dissected and weighed. Serum levels of leptin, IGF-1, and testosterone were determined using ELISA kits. Serum levels of isoflavones were assayed by GC/MS. Liver activity of selenium-dependent glutathione peroxidase 1 was measured as an indicator of selenium status. Serum isoflavone concentrations were nearly 100-fold higher at 35 days of age (1187.1 vs. 14.4 ng/mL, mean ± SD) in pups fed the high vs. low isoflavone diets, and remained so at 100 and 200 days, and in breeders. There were no dietary differences in liver glutathione peroxidase activity in pups or breeders. High isoflavone intake significantly (p = 0.001–0.047) reduced body weight in rat pups from 35 days onward, but not in breeders. Body fat and leptin were likewise significantly reduced by high isoflavones in pups while effects in breeders were less pronounced but still significant. High intake of Se and isoflavones each decreased serum IGF-1 in pups at 100 and 200 days, but not in breeders. No consistent dietary effects were observed on serum testosterone or relative weights of prostates. In pups, the combination of high isoflavones and high selenium produced the lowest weight gain, the lowest serum leptin, and the lowest serum IGF-1 concentrations of all four diets. Combined intake of high selenium and high isoflavones may achieve greater chemopreventive effects than either compound individually. The timing of supplementation may determine the significance of its effects.
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影响因子：
7.1
作者：
Barnard, R. James
通讯作者：
Barnard, R. James
影响因子：
5.6
作者：
CHRISTENSEN, MJ;CAMMACK, PM;WRAY, CD
通讯作者：
WRAY, CD
影响因子：
4
作者：
Culig, Zoran;Bartsch, Georg
通讯作者：
Bartsch, Georg
影响因子：
5.7
作者：
Klein, SL;Wisniewski, AB;Gearhart, JP
通讯作者：
Gearhart, JP
DOI：
10.1016/j.urolonc.2007.01.016
发表时间：
2008-03-01
影响因子：
2.7
作者：
Grainger, Elizabeth M.;Kim, H. Sunny;Clinton, Steven K.
通讯作者：
Clinton, Steven K.