Ancestral archaea expanded the genetic code with pyrrolysine.
Ancestral archaea expanded the genetic code with pyrrolysine.
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祖先古细菌用吡咯赖氨酸扩展了遗传密码
DOI：
10.1016/j.jbc.2022.102521
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发表时间：
2022-11
影响因子：
4.8
通讯作者：
Tharp, Jeffery M.
中科院分区：
文献类型：
--
作者：
Guo, Li -Tao;Amikura, Kazuaki;Jiang, Han -Kai;Mukai, Takahito;Fu, Xian;Wang, Yane-Shih;O'Donoghue, Patrick;Soell, Dieter;Tharp, Jeffery M.
吡咯基-TRNA合成酶（PYLRS）促进了22nd氨基酸吡咯氨氨酸的共转运安装。在活细胞中类（ΔPylsn）缺乏在菌群中广泛保守的N末端tRNA结合结构域，但在细菌和真核生物中保持活性和正交。和甲基甲基酚的ΔPylsn类塔的底物谱。来自甲壳虫的全长酶，CA。 ）序列的古细菌和细菌。在生命的三个结构域的演变之前，对塔的变化特征的演变提前限制。 。扩张。
The pyrrolysyl-tRNA synthetase (PylRS) facilitates the cotranslational installation of the 22nd amino acid pyrrolysine. Owing to its tolerance for diverse amino acid substrates, and its orthogonality in multiple organisms, PylRS has emerged as a major route to install noncanonical amino acids into proteins in living cells. Recently, a novel class of PylRS enzymes was identified in a subset of methanogenic archaea. Enzymes within this class (ΔPylSn) lack the N-terminal tRNA-binding domain that is widely conserved amongst PylRS enzymes, yet remain active and orthogonal in bacteria and eukaryotes. In this study, we use biochemical and in vivo UAG-readthrough assays to characterize the aminoacylation efficiency and substrate spectrum of a ΔPylSn class PylRS from the archaeon Candidatus Methanomethylophilus alvus. We show that, compared with the full-length enzyme from Methanosarcina mazei, the Ca. M. alvus PylRS displays reduced aminoacylation efficiency but an expanded amino acid substrate spectrum. To gain insight into the evolution of ΔPylSn enzymes, we performed molecular phylogeny using 156 PylRS and 105 pyrrolysine tRNA (tRNAPyl) sequences from diverse archaea and bacteria. This analysis suggests that the PylRS•tRNAPyl pair diverged before the evolution of the three domains of life, placing an early limit on the evolution of the Pyl-decoding trait. Furthermore, our results document the coevolutionary history of PylRS and tRNAPyl and reveal the emergence of tRNAPyl sequences with unique A73 and U73 discriminator bases. The orthogonality of these tRNAPyl species with the more common G73-containing tRNAPyl will enable future efforts to engineer PylRS systems for further genetic code expansion.
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影响因子：
4.5
作者：
Cozannet M;Borrel G;Roussel E;Moalic Y;Allioux M;Sanvoisin A;Toffin L;Alain K
通讯作者：
Alain K
DOI：
10.1128/genomea.00453-13
发表时间：
2013-07-11
期刊：
Genome announcements
影响因子：
--
作者：
Borrel G;Harris HM;Parisot N;Gaci N;Tottey W;Mihajlovski A;Deane J;Gribaldo S;Bardot O;Peyretaillade E;Peyret P;O'Toole PW;Brugère JF
通讯作者：
Brugère JF
影响因子：
4.8
作者：
Jiang, Ruisheng;Krzycki, Joseph A.
通讯作者：
Krzycki, Joseph A.
影响因子：
14.9
作者：
Chen, I-Min A.;Chu, Ken;Kyrpides, Nikos C.
通讯作者：
Kyrpides, Nikos C.
DOI：
10.1099/ijs.0.65049-0
发表时间：
2007-12-01
影响因子：
2.8
作者：
Cheng, Lei;Qiu, Tian-Lei;Zhang, Hui
通讯作者：
Zhang, Hui