A DBHS family member regulates male determination in the filariasis vector Armigeres subalbatus.
A DBHS family member regulates male determination in the filariasis vector Armigeres subalbatus.
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DOI：
10.1038/s41467-023-37983-y
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发表时间：
2023-04-21
影响因子：
16.6
通讯作者：
Chen, Xiao-Guang
中科院分区：
文献类型：
--
作者：
Liu, Peiwen;Yang, Wenqiang;Kong, Ling;Zhao, Siyu;Xie, Zhensheng;Zhao, Yijie;Wu, Yang;Guo, Yijia;Xie, Yugu;Liu, Tong;Jin, Binbin;Gu, Jinbao;Tu, Zhijian Jake;James, Anthony A.;Chen, Xiao-Guang
昆虫之间控制性别决定的初始信号差异很大。在这里，我们表明，Armigeres subalbatus M 因子（AsuMf）是果蝇行为/人类剪接（DBHS）基因家族常染色体基因的雄性特异性重复，是人类丝虫病媒介蚊子 Ar 性别决定的潜在主要信号。亚巴图斯。我们的结果表明 AsuMf 满足 M 因子的两个基本要求：雄性特异性表达和早期胚胎表达。 AsuMf 的消融导致双性和无结果的雄性特异性剪接转变为雌性特异性剪接，从而导致雄性在形态和一般转录谱上女性化。这些数据支持这样的结论：AsuMf 对于 Ar 中的男性发育至关重要。 subalbatus 并揭示了一个雄性决定因素，该因素源自保守 DBHS 家族成员的复制和随后的新功能化。 Armigeres subalbatus 性别决定基因座 M 映射到第三染色体，但其身份未知。在这里，他们鉴定了一种性别特异性基因 AsuMf，该基因对于男性发育至关重要，并表明它是一种新的男性决定因素，源自保守的 DBHS 家族成员的复制和新功能化。
The initial signals governing sex determination vary widely among insects. Here we show that Armigeres subalbatus M factor (AsuMf), a male-specific duplication of an autosomal gene of the Drosophila behaviour/human splicing (DBHS) gene family, is the potential primary signal for sex determination in the human filariasis vector mosquito, Ar. subalbatus. Our results show that AsuMf satisfies two fundamental requirements of an M factor: male-specific expression and early embryonic expression. Ablations of AsuMf result in a shift from male- to female-specific splicing of doublesex and fruitless, leading to feminization of males both in morphology and general transcription profile. These data support the conclusion that AsuMf is essential for male development in Ar. subalbatus and reveal a male-determining factor that is derived from duplication and subsequent neofunctionalization of a member of the conserved DBHS family. The Armigeres subalbatus sex-determining locus, M, maps to the 3rd chromosome, but its identity was unknown. Here they identify a sex-specific gene, AsuMf, that is essential for male development and show that it is a novel male-determining factor derived from duplication and neofunctionalization of a conserved DBHS family member.
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影响因子：
7.7
作者：
Criscione, Frank;Qi, Yumin;Tu, Zhijian
通讯作者：
Tu, Zhijian
影响因子：
7
作者：
Ferdig, MT;Taft, AS;Christensen, BM
通讯作者：
Christensen, BM
影响因子：
1.2
作者：
Juhn, Jennifer;James, Anthony A.
通讯作者：
James, Anthony A.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z