Migration of CD11b+ accessory cells during murine lung regeneration.
Migration of CD11b+ accessory cells during murine lung regeneration.
复制标题
DOI：
10.1016/j.scr.2012.12.006
复制
发表时间：
2013-05
影响因子：
1.2
通讯作者：
Mentzer, Steven J.
中科院分区：
文献类型：
--
作者：
Chamoto, Kenji;Gibney, Barry C.;Lee, Grace S.;Ackermann, Maximilian;Konerding, Moritz A.;Tsuda, Akira;Mentzer, Steven J.
在许多哺乳动物物种中，切除一个肺会导致剩余的肺生长到接近基线水平。在研究肺切除术后的小鼠中，我们使用形态学测量来证明肺切除术后21天内的新肺泡形成。值得注意的是，在此期间的详细组织学检查显示无显著肺部炎症。为了鉴定隐性血源性细胞，我们使用了肺切除术后肺生长的联体模型（野生型/GFP）。肺切除术后肺巨噬细胞的流式细胞术显示表达造血膜分子CD 11b的细胞数量迅速增加;整个GFP+群体中有64.5%为CD 11b+。荧光显微镜显示，CD 11b+外周血细胞迁移到间质组织和肺泡气室。在CD 11b缺陷的小鼠（CD 18-/-突变体）中进行的肺切除术显示几乎不存在白细胞迁移到气腔室（p<.001），并且肺重量（p<.05）和肺体积（p<.05）显示肺生长受损。分配的CD 11b+细胞的转录活性显示，肺泡气隙中Angpt 1、II 1b和Mmp 8、Mmp 9、Ncam 1、Sele、Sell、Selp的转录显著增加，间质组织中Adamts 2、Ecm 1、Egf、Mmp 7、Npr 1、Tgfb 2的转录显著增加（>4倍调节; p<0.05）。这些数据表明，血液传播的CD 11b+细胞代表辅助细胞的人口有助于肺切除术后肺生长。
In many mammalian species, the removal of one lung leads to growth of the remaining lung to near-baseline levels. In studying post-pneumonectomy mice, we used morphometric measures to demonstrate neoalveolarization within 21 days of pneumonectomy. Of note, the detailed histology during this period demonstrated no significant pulmonary inflammation. To identify occult blood-borne cells, we used a parabiotic model (wild-type/GFP) of post-pneumonectomy lung growth. Flow cytometry of post-pneumonectomy lung digests demonstrated a rapid increase in the number of cells expressing the hematopoietic membrane molecule CD11b; 64.5% of the entire GFP+ population were CD11b+. Fluorescence microscopy demonstrated that the CD11b+ peripheral blood cells migrated into both the interstitial tissue and alveolar airspace compartments. Pneumonectomy in mice deficient in CD11b (CD18-/- mutants) demonstrated near-absent leukocyte migration into the airspace compartment (p<.001) and impaired lung growth as demonstrated by lung weight (p<.05) and lung volume (p<.05). Transcriptional activity of the partitioned CD11b+ cells demonstrated significantly increased transcription of Angpt1, Il1b, and Mmp8, Mmp9, Ncam1, Sele, Sell, Selp in the alveolar airspace and Adamts2, Ecm1, Egf, Mmp7, Npr1, Tgfb2 in the interstitial tissue (>4-fold regulation; p<.05). These data suggest that blood-borne CD11b+ cells represent a population of accessory cells contributing to post-pneumonectomy lung growth.
登录
查看更多内容
对抗生素小鼠的跨循环和细胞分布动力学。
影响因子：
5.6
作者：
Gibney, Barry C.;Chamoto, Kenji;Lee, Grace S.;Simpson, Dinee C.;Miele, Lino F.;Tsuda, Akira;Konerding, Moritz A.;Wagers, Amy;Mentzer, Steven J.
通讯作者：
Mentzer, Steven J.
影响因子：
4.6
作者：
Hiesmayr, MJ;Spittler, A;Roth, E
通讯作者：
Roth, E
影响因子：
20.3
作者：
Fantin, Alessandro;Vieira, Joaquim M.;Ruhrberg, Christiana
通讯作者：
Ruhrberg, Christiana
DOI：
10.1165/rcmb.2006-0330oc
发表时间：
2007-04-01
影响因子：
6.4
作者：
Matthews, Kate E.;Karabeg, Adela;Ronchese, Franca
通讯作者：
Ronchese, Franca
影响因子：
5.8
作者：
Lin M;Chamoto K;Gibney BC;Lee GS;Collings-Simpson D;Houdek J;Konerding MA;Tsuda A;Mentzer SJ
通讯作者：
Mentzer SJ