Large scale in vitro propagation of Anoectochilus roxburghii for commercial application: Pharmaceutically important and ornamental plant
Large scale in vitro propagation of Anoectochilus roxburghii for commercial application: Pharmaceutically important and ornamental plant
复制标题
金线莲的大规模体外繁殖用于商业应用：药用和观赏植物
DOI：
10.1016/j.indcrop.2015.03.032
复制
发表时间：
2015-08
影响因子：
5.9
通讯作者：
Mingyan Li
中科院分区：
文献类型：
--
作者：
Qingsong Shao;Mengjie Xu;Wangshu Zhang;Mingyan Li
金线莲（Anoectochilus roxburghii）是兰科重要的药用和观赏植物，由于过度采集和适宜生境的丧失而濒临灭绝。A.已经测试了刺梨的微繁殖，但是没有一种提供用于大规模微繁殖的有效方法。本研究从不定芽的发生与分化、不定芽的增殖、不定根的诱导和驯化等方面探讨了一种改良的高效繁殖方法。从田间生长的植株上切下的节段作为外植体。结果表明，MS +1.5 mg l−16-苄基腺嘌呤（BA）是最适宜的不定芽诱导培养基，不定芽诱导率为91.67%。将芽接种在含有不同浓度和组合的细胞分裂素BA（0.5-4.0 mg l−1）、激动素（Kn）（0.1-2.0 mg l −1）和生长素1-α-萘乙酸（NAA）（0-0.5 mg l − 1）的半强度MS、MS、Vacin and Went（VW）和Gamborg（B5）培养基上，以选择芽生长和增殖的最佳条件。其中，在附加3.0 mg l−1BA、1.0 mg l− 1 Kn、0.5 mg l− 1 NAA和其他添加剂的半浓度MS培养基上增殖率最高，为4.33。结果表明：1/2 MS +0.6 mg l − 1 NAA +0.3 mg l− 1 IBA +100 mg l− 1香蕉泥为最佳生根培养基，生根率达93.33%。将生根的小植株成功地转移到含有1：2比例的无菌砂和泥炭土混合物的塑料杯中，并且表面覆盖有活苔藓。A.刺梨为90.2%。该方法可有效地用于该濒危药用观赏植物种质资源的大规模繁殖和保存。
Anoectochilus roxburghii(Orchidaceae family) is an important medicinal and ornamental plant which is in extinct condition because of the over-collected and the loss of its suitable habitats. Several protocols forA. roxburghiimicropropagation have been tested but none has provided an effective method for large-scale micropropagation. The study investigates an improved and efficient propagation method including shoots generation and formation, shoot proliferation, inducing root and acclimatization. The nodal segments cut from field-grown plants were adopted as explants. Half-strength Murashige and Skoog (MS) medium supplemented with 1.5 mg l−16-benzyladenine (BA) was proved to be the optimal medium for shoots generation and induction, on which, the shoot formation rate was 91.67%. Shoots were inoculated on such four media as half-strength MS, MS, Vacin and Went (VW), and Gamborg (B5), containing various concentrations and combinations of the cytokinins BA, 0.5–4.0 mg l−1, kinetin (Kn), 0.1–2.0 mg l−1, and the auxin 1-α-naphthaleneacetic acid (NAA), 0–0.5 mg l−1, to select the optimal conditions for shoot growth and proliferation. Among those, the highest proliferation rate was 4.33, which was obtained on half-strength MS medium fortified with 3.0 mg l−1BA, 1.0 mg l−1Kn, 0.5 mg l−1NAA and additives. The half-strength MS medium supplemented with 0.6 mg l−1NAA, 0.3 mg l−1indole-3-butyric acid (IBA) and 100 mg l−1banana mashes turned out to be best medium forin vitrorooting, which has a inducing root rate of 93.33%. The rooted plantlets were successfully transferred into plastic cups containing a sterile sand and peat soil mixture in a ratio of 1:2, and the surface was covered with live moss. The survival rate forA. roxburghiiwas 90.2%. The described protocol can be efficiently used for the large-scale propagation and conservation of the germplasm of this endangered medicinal and ornamental plant.
登录
查看更多内容
影响因子：
5.9
作者：
M. Thiyagarajan;P. Venkatachalam
通讯作者：
M. Thiyagarajan;P. Venkatachalam
影响因子：
5.7
作者：
M. S. Rathore;M. S. Rathore;N. S. Shekhawat
通讯作者：
M. S. Rathore;M. S. Rathore;N. S. Shekhawat
DOI：
10.4268/cjcmm20140506
发表时间：
2014-03
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
Qing-song Shao;Aicun Zhou;Run-huai Hu;Yu-Yun Zhang;Tanglong Liu;Ming-yan Li
通讯作者：
Qing-song Shao;Aicun Zhou;Run-huai Hu;Yu-Yun Zhang;Tanglong Liu;Ming-yan Li
影响因子：
3.1
作者：
O. O. Fajinmi-O.;S. Amoo;J. F. Finnie;J. Staden
通讯作者：
O. O. Fajinmi-O.;S. Amoo;J. F. Finnie;J. Staden
DOI：
10.1007/bf02822700
发表时间：
1996-10
期刊：
In Vitro Cellular & Developmental Biology - Plant
影响因子：
--
作者：
T. Gaspar;C. Kevers;C. Penel;H. Greppin;D. M. Reid;T. Thorpe
通讯作者：
T. Gaspar;C. Kevers;C. Penel;H. Greppin;D. M. Reid;T. Thorpe