Circular RNA YAP1 inhibits the proliferation and invasion of gastric cancer cells by regulating the miR-367-5p/p27 (Kip1) axis.
Circular RNA YAP1 inhibits the proliferation and invasion of gastric cancer cells by regulating the miR-367-5p/p27 (Kip1) axis.
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环状RNA YAP1通过调控miR-367-5p/p27 Kip1轴抑制胃癌细胞增殖和侵袭
DOI：
10.1186/s12943-018-0902-1
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发表时间：
2018-10-18
期刊：
影响因子：
37.3
通讯作者：
Zhu J
中科院分区：
文献类型：
--
作者：
Liu H;Liu Y;Bian Z;Zhang J;Zhang R;Chen X;Huang Y;Wang Y;Zhu J
背景：环状rna （Circular RNAs, circRNAs）是一种新型非编码rna，其在胃癌（GC）中的功能尚不清楚。最近的研究表明，circRNAs在癌症发展和某些类型的病理反应中发挥重要作用，作为microRNA （miRNA）海绵调节基因表达。方法：使用CircNet筛选潜在的circrna，并通过实时荧光定量PCR （qRT-PCR）和FISH分别在17个GC组织和80个配对GC组织中验证circYAP1的表达水平。通过CircYAP1过表达和敲低实验，评估CircYAP1在体外和体内的作用，并通过RNA体内沉淀实验、western blotting、荧光素酶实验和救援实验验证其分子机制。结果：CircYAP1在胃癌组织中的表达水平明显低于癌旁正常组织，且CircYAP1低表达胃癌患者的生存时间较CircYAP1高表达胃癌患者短。功能上，circYAP1过表达抑制体外和体内细胞生长和侵袭，但其敲低逆转了这些作用。进一步分析表明circYAP1海绵miR-367-5p抑制p27kip1表达和GC进展。结论：我们的研究结果表明，circYAP1通过靶向miR-367-5p/ p27kip1轴，在胃癌细胞中发挥肿瘤抑制作用，并可能提供胃癌患者生存的预后指标。
Background:Circular RNAs (circRNAs) are a new type of non-coding RNAs and their functions in gastric cancer (GC) remain unclear. Recent studies have revealed that circRNAs play an important role in cancer development and certain types of pathological responses, acting as microRNA (miRNA) sponges to regulate gene expression.Methods:CircNet was used to screen potential circRNAs and validated circYAP1 expression levels in 17 GC tissues by quantitative real-time PCR (qRT-PCR) and another 80 paired GC tissues by FISH. CircYAP1 overexpression and knockdown experiments were conducted to assess the effects of circYAP1 in vitro and in vivo, and its molecular mechanism was demonstrated by RNA in vivo precipitation assays, western blotting, luciferase assay and rescue experiments.Results:CircYAP1 expression level was significantly lower in GC tissues than the adjacent normal tissues, and GC patients with circYAP1 low expression had shorter survival times as compared with those with circYAP1 high expression. Functionally, circYAP1 overexpression inhibited cell growth and invasion in vitro and in vivo, but its knockdown reversed these effects. Further analysis showed that circYAP1 sponged miR-367-5p to inhibit p27Kip1expression and GC progression.Conclusion:Our findings demonstrate that circYAP1 functions as a tumor suppressor in GC cells by targeting the miR-367-5p/p27Kip1axis and may provide a prognostic indicator of survival in GC patients.
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影响因子：
--
作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
通讯作者：
Zhou Y
影响因子：
4.6
作者：
Barrett, Steven P.;Salzman, Julia
通讯作者：
Salzman, Julia
影响因子：
4.1
作者：
Dudekulay, Dawood B.;Panda, Amaresh C.;Gorospe, Myriam
通讯作者：
Gorospe, Myriam
miRNA在癌细胞和肿瘤微环境中的管制。
影响因子：
28.2
作者：
Rupaimoole R;Calin GA;Lopez-Berestein G;Sood AK
通讯作者：
Sood AK
影响因子：
37.3
作者：
Kang W;Tong JH;Lung RW;Dong Y;Zhao J;Liang Q;Zhang L;Pan Y;Yang W;Pang JC;Cheng AS;Yu J;To KF
通讯作者：
To KF