NilD CRISPR RNA contributes to Xenorhabdus nematophila colonization of symbiotic host nematodes.
NilD CRISPR RNA contributes to Xenorhabdus nematophila colonization of symbiotic host nematodes.
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DOI：
10.1111/mmi.12715
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发表时间：
2014-09
影响因子：
3.6
通讯作者：
Goodrich-Blair H
中科院分区：
文献类型：
--
作者：
Veesenmeyer JL;Andersen AW;Lu X;Hussa EA;Murfin KE;Chaston JM;Dillman AR;Wassarman KM;Sternberg PW;Goodrich-Blair H
细菌嗜黄杆菌是昆虫病原性Steinernema carpocapsae线虫的互利互惠者，并促进昆虫宿主的感染。X.嗜水气单胞菌定殖于S.在昆虫之间传播在X.将转座子插入到缺乏明显编码潜力的区域。我们证明转座子破坏了单个CRISPR RNA（NilD RNA）的表达。一种变体NilD RNA也由X.从S. anatoliense和S.韦伯斯特线虫只有零个来自S。carpocapsae株系。嗜线虫拯救了nilD 6：：Tn 5突变体的定殖缺陷，并且该突变体在定殖所有三种线虫宿主物种中是有缺陷的。NilD表达取决于相关Cas 6 e而不是Cas 3（I-E型CRISPR相关机制的组分）的存在。虽然cas 6 e在补充菌株中的缺失废除了线虫定殖，但其在野生型亲本中的破坏没有。同样地，亲本菌株中的nilD缺失不影响线虫的定殖，揭示了对NilD的需要仅在某些遗传背景中是明显的。我们的数据表明，NilD RNA是互惠宿主定殖的条件所必需的，并表明它的功能是调节内源性基因表达。
The bacterium Xenorhabdus nematophila is a mutualist of entomopathogenic Steinernema carpocapsae nematodes and facilitates infection of insect hosts. X. nematophila colonizes the intestine of S. carpocapsae which carries it between insects. In the X. nematophila colonization-defective mutant nilD6::Tn5, the transposon is inserted in a region lacking obvious coding potential. We demonstrate that the transposon disrupts expression of a single CRISPR RNA, NilD RNA. A variant NilD RNA also is expressed by X. nematophila strains from S. anatoliense and S. websteri nematodes. Only nilD from the S. carpocapsae strain of X. nematophila rescued the colonization defect of the nilD6::Tn5 mutant, and this mutant was defective in colonizing all three nematode host species. NilD expression depends on the presence of the associated Cas6e but not Cas3, components of the Type I-E CRISPR-associated machinery. While cas6e deletion in the complemented strain abolished nematode colonization, its disruption in the wild-type parent did not. Likewise, nilD deletion in the parental strain did not impact colonization of the nematode, revealing that the requirement for NilD is evident only in certain genetic backgrounds. Our data demonstrate that NilD RNA is conditionally necessary for mutualistic host colonization and suggest that it functions to regulate endogenous gene expression.
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影响因子：
3.4
作者：
Chaston JM;Murfin KE;Heath-Heckman EA;Goodrich-Blair H
通讯作者：
Goodrich-Blair H
影响因子：
8
作者：
Gasiunas, Giedrius;Sinkunas, Tomas;Siksnys, Virginijus
通讯作者：
Siksnys, Virginijus
影响因子：
30.8
作者：
Jansen, G;Thijssen, KL;Plasterk, RHA
通讯作者：
Plasterk, RHA
影响因子：
3.2
作者：
Deveau, Helene;Barrangou, Rodolphe;Moineau, Sylvain
通讯作者：
Moineau, Sylvain
影响因子：
4.4
作者：
Forst, SA;Tabatabai, N
通讯作者：
Tabatabai, N