Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice
Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice
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Thermo-TRP 和肠道微生物群参与肥胖小鼠低温暴露期间的产热和能量代谢
DOI：
10.1242/jeb.218974
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发表时间：
2020-01
影响因子：
2.8
通讯作者：
Wang Dehua
中科院分区：
文献类型：
--
作者：
Wen Jing;Bo Tingbei;Zhang Xueying;Wang Zuoxin;Wang Dehua
摘要环境温度和食物成分会影响宿主的能量代谢。热瞬时受体电位离子通道（thermo-TRP）可以检测温度信号，参与产热和能量稳态的调节。此外，肠道微生物群也与产热和肥胖有关。在本研究中，我们测试了热TRP和肠道微生物群参与减少低温暴露期间饮食诱导的肥胖（DIO）的假设。将肥胖（体重增加> 45%）、瘦（体重增加<15%）和对照（体重增加<1%）组中的C57 BL/6J小鼠暴露于高（23 ± 1 ° C）或低（4 ± 1 ° C）环境温度28天。 我们的数据表明，低温暴露减弱DIO，但增强棕色脂肪组织（BAT）产热。低温暴露还导致下丘脑中去甲肾上腺素（NA）浓度增加，小肠中TRP melastatin 8（TRPM8）表达减少，以及肠道微生物群的组成和多样性改变。在DIO小鼠中，总能量摄入沿着减少，同时小肠中TRP锚蛋白1（TRPA 1）表达减少和NA浓度增加。DIO小鼠的粪便样本中，属水平上的颤螺旋菌、[反刍球菌]、乳球菌和克里斯滕森氏菌数量也有所增加，而普雷沃氏菌、臭杆菌和乳杆菌数量则有所减少。总之，我们的数据表明，热TRP和肠道微生物群参与DIO小鼠在低温暴露期间的产热和能量代谢。总结：Thermo-TRP和肠道微生物群参与减轻C57BL/6J小鼠低温暴露期间饮食诱导的肥胖。
ABSTRACT Ambient temperature and food composition can affect energy metabolism of the host. Thermal transient receptor potential ion channels (thermo-TRPs) can detect temperature signals and are involved in the regulation of thermogenesis and energy homeostasis. Further, the gut microbiota have also been implicated in thermogenesis and obesity. In the present study, we tested the hypothesis that thermo-TRPs and gut microbiota are involved in reducing diet-induced obesity (DIO) during low temperature exposure. C57BL/6J mice in obese (body mass gain >45%), lean (body mass gain <15%) and control (body mass gain <1%) groups were exposed to high (23±1°C) or low (4±1°C) ambient temperature for 28 days. Our data showed that low temperature exposure attenuated DIO, but enhanced brown adipose tissue (BAT) thermogenesis. Low temperature exposure also resulted in increased noradrenaline (NA) concentrations in the hypothalamus, decreased TRP melastatin 8 (TRPM8) expression in the small intestine, and altered composition and diversity of gut microbiota. In DIO mice, there was a decrease in overall energy intake along with a reduction in TRP ankyrin 1 (TRPA1) expression and an increase in NA concentration in the small intestine. DIO mice also showed increases in Oscillospira, [Ruminococcus], Lactococcus and Christensenella and decreases in Prevotella, Odoribacter and Lactobacillus at the genus level in fecal samples. Together, our data suggest that thermos-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure in DIO mice. Summary: Thermo-TRPs and gut microbiota are involved in attenuating diet-induced obesity during low temperature exposure in C57BL/6J mice.
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影响因子：
64.8
作者：
HERVEY, GR
通讯作者：
HERVEY, GR
影响因子：
1.6
作者：
Iwasaki, Yusaku;Tanabe, Manabu;Watanabe, Tatsuo
通讯作者：
Watanabe, Tatsuo
DOI：
--
发表时间：
2017
期刊：
--
影响因子：
--
作者：
Dossus;Barquera;Blottière;Franks;Gunter;Hwalla;Leitzmann;Margetts;Nishida;Potischman;Stepien;Winichagoon;Wiseman;Willett
通讯作者：
Dossus;Barquera;Blottière;Franks;Gunter;Hwalla;Leitzmann;Margetts;Nishida;Potischman;Stepien;Winichagoon;Wiseman;Willett
影响因子：
4.1
作者：
Rossato, Marco;Granzotto, Marnie;Vettor, Roberto
通讯作者：
Vettor, Roberto
微生物群耗竭损害棕色脂肪组织的产热和白色脂肪组织的褐变
影响因子：
8.8
作者：
Li, Baoguo;Li, Li;Speakman, John R.
通讯作者：
Speakman, John R.