Mechanical outcome of accelerated corneal crosslinking evaluated by Brillouin microscopy.
Mechanical outcome of accelerated corneal crosslinking evaluated by Brillouin microscopy.
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DOI：
10.1016/j.jcrs.2017.07.037
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发表时间：
2017-11
影响因子：
2.8
通讯作者：
Scarcelli G
中科院分区：
文献类型：
--
作者：
Webb JN;Su JP;Scarcelli G
目的：利用布里渊显微镜定量分析不同UV-A强度和暴露时间的CXL手术引起的角膜力学变化。马里兰州大学，College Park，MD实验室研究将猪角膜样品清除上皮细胞并用0.1%核黄素溶液浸泡。接下来，将样品暴露于具有不同强度和暴露时间的临床标准5.4J/cm 2的UV-A辐射：3 mW/cm 2持续30分钟，9 mW/cm 2持续10分钟，34 mW/cm 2持续2.65分钟，以及50 mW/cm 2持续1.80分钟。使用布里渊显微镜，计算每个角膜样品的布里渊模量作为辐射强度/暴露时间的函数。为了验证，使用压缩应力-应变测试，找到杨氏模量，并对每个辐照条件进行比较。与9（p ≤ 0.05）、34（p ≤ 0.01）和50 mW/cm 2条件（p ≤ 0.01）相比，标准3 mW/cm 2辐照条件产生的角膜布里渊模量增加显著更大。深度分析显示，标准和9 mW/cm 2条件下的前部相似，但9 mW/cm 2样品的中央和后部硬化程度明显低于标准。在34和50 mW/cm 2条件下的所有切片中，标准方案的硬化显著更大（p ≤ 0.01）。对于所有样品，布里渊散射衍生的布里渊散射模量的总体变化与杨氏模量的增加相关（R2 = 0.98）。虽然UV-A光剂量保持恒定，但加速照射过程会降低CXL硬化的有效性。具体地，布里渊分析揭示了加速协议在角膜的较深部分中尤其无效。
To use Brillouin microscopy to quantify corneal mechanical changes induced by CXL procedures of different UV-A intensity and exposure time. University of Maryland, College Park, MD Laboratory Study Porcine cornea samples were debrided of epithelia and soaked with 0.1% riboflavin solution. Next, the samples were exposed to a clinically-standard 5.4 J/cm2 of UV-A radiation with varying intensity and exposure time: 3 mW/cm2 for 30 minutes, 9 mW/cm2 for 10 minutes, 34 mW/cm2 for 2.65 minutes, and 50 mW/cm2 for 1.80 minutes. Using Brillouin microscopy, the Brillouin modulus for each cornea sample was computed as a function of radiation intensity/exposure time. For validation, using a compressive stress-strain test, the Young’s Modulus was found and compared for each irradiation condition. The standard 3 mW/cm2 irradiance condition produced a significantly larger increase in corneal Brillouin modulus compared to the 9 (p ≤ 0.05), 34 (p ≤ 0.01), and 50 mW/cm2 conditions (p ≤ 0.01). Depth-analysis revealed similar anterior sections of the standard and 9 mW/cm2 conditions, but significantly less stiffening in the central and posterior of the 9 mW/cm2 sample than the standard. The stiffening of the standard protocol was significantly larger (p ≤ 0.01) in all sections of the 34 and 50 mW/cm2 conditions. For all samples, the overall change in Brillouin-derived Brillouin modulus correlated to the increase in Young’s Modulus (R2 = 0.98). Although the UV-A light dose was kept constant, accelerating the irradiation process decreased the effectiveness of CXL stiffening. Specifically, Brillouin analysis reveals that accelerated protocols are especially ineffective in the deeper portions of the cornea.
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