Purinoceptor P2K1/DORN1 Enhances Plant Resistance Against a Soilborne Fungal Pathogen, Rhizoctonia solani.
Purinoceptor P2K1/DORN1 Enhances Plant Resistance Against a Soilborne Fungal Pathogen, Rhizoctonia solani.
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DOI：
10.3389/fpls.2020.572920
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发表时间：
2020
影响因子：
5.6
通讯作者：
Tanaka K
中科院分区：
文献类型：
--
作者：
Kumar S;Tripathi D;Okubara PA;Tanaka K
嘌呤受体P2K1/DORN1识别细胞外的ATP，这是一种损伤相关的分子模式(DAMP)，当细胞受到损伤和坏死破坏时释放出来，进而提高植物的先天免疫。已知的是，P2K1使植物对叶部生物营养、半生物营养和死亡营养病原体产生抗性。然而，到目前为止，还没有关于它在抵御根部病原菌方面的功能的信息。在本报告中，我们描述了P2K1在拟南芥对水稻纹枯病菌(Rhizoctonia Solani)抗性中的作用。在盆栽试验中，在病原菌存在的情况下，拟南芥P2K1高表达系OxP2K1表现出比野生型更长的根长和更大的玫瑰花结面积。相反，在呼吸爆发复合体NADPH氧化酶的两个亚基中存在缺陷的敲除突变体dorn1-3和双突变体rbohd/f，在病原菌存在时，与野生型相比，其地上部和根长以及莲座丛表面积显著减少。与野生型相比，根中PR1、PDF1.2和JAZ5的表达在dorn1-3和rbohd/f中降低，而在OxP2K1中表达升高，表明水杨酸和茉莉酸防御信号通路在抗性中起作用。这些结果表明，潮湿介导的防御系统对一种重要的根部坏死性真菌病原菌具有基础抗性。
The purinoceptor P2K1/DORN1 recognizes extracellular ATP, a damage-associated molecular pattern (DAMP) released upon cellular disruption by wounding and necrosis, which in turn, boost plant innate immunity. P2K1 is known to confer plant resistance to foliar biotrophic, hemi-biotrophic, and necrotrophic pathogens. However, until now, no information was available on its function in defense against root pathogens. In this report, we describe the contribution of P2K1 to resistance in Arabidopsis against Rhizoctonia solani, a broad host range, necrotrophic soilborne fungal pathogen. In pot assays, the Arabidopsis P2K1 overexpression line OxP2K1 showed longer root length and a greater rosette surface area than wild type in the presence of the pathogen. In contrast, the knockout mutant dorn1-3 and the double mutant rbohd/f, defective in two subunits of the respiratory burst complex NADPH oxidase, exhibited significant reductions in shoot and root lengths and rosette surface area compared to wild type when the pathogen was present. Expression of PR1, PDF1.2, and JAZ5 in the roots was reduced in dorn1-3 and rbohd/f and elevated in OxP2K1 relative to wild type, indicating that the salicylate and jasmonate defense signaling pathways functioned in resistance. These results indicated that a DAMP-mediated defense system confers basal resistance against an important root necrotrophic fungal pathogen.
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