Social and environmental transmission spread different sets of gut microbes in wild mice

Social and environmental transmission spread different sets of gut microbes in wild mice
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社会和环境传播在野生小鼠中传播了不同的肠道微生物

DOI:
10.1101/2023.07.20.549849
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发表时间:
2023
期刊:
--
影响因子:
--
通讯作者:
Raulo A
Raulo A
中科院分区:
--
文献类型:
--
作者:
Raulo A

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肠道微生物塑造了生物体生物学的许多方面,但这些关键细菌如何在自然种群中的宿主之间传播仍然知之甚少。最近在哺乳动物中的研究强调通过社会接触传播或通过环境接触间接传播,但尚未直接评估不同途径的相对重要性。在这里,我们使用了一种新的基于射频识别的跟踪系统来收集野生小鼠(Apodemus sylvaticus)的社会关系,空间使用和微生境的长期高分辨率数据,同时定期用16S核糖体RNA分析来表征它们的肠道微生物群。通过对所得数据的概率建模,我们分别识别出由社交网络捕获的社会和环境传播的积极和统计上不同的信号,以及家庭范围的重叠。引人注目的是,具有不同生物属性的微生物驱动了这些不同的传输信号。虽然社会网络对微生物群的影响是由厌氧细菌驱动的,但共享空间的影响最受耐氧孢子形成细菌的影响。这些发现支持了这样的预测,即社会接触对于耐氧性低的微生物的转移很重要,而那些能够耐受氧气或形成孢子的微生物可能能够通过环境间接传播。总的来说,这些结果表明,社会和环境传播途径可以传播哺乳动物肠道微生物群的生物学上不同的成员。
Gut microbes shape many aspects of organismal biology, yet how these key bacteria transmit among hosts in natural populations remains poorly understood. Recent work in mammals has emphasized either transmission through social contacts or indirect transmission through environmental contact, but the relative importance of different routes has not been directly assessed. Here we used a novel radio-frequency identification-based tracking system to collect long-term high-resolution data on social relationships, space use and microhabitat in a wild population of mice (Apodemus sylvaticus), while regularly characterizing their gut microbiota with 16S ribosomal RNA profiling. Through probabilistic modelling of the resulting data, we identify positive and statistically distinct signals of social and environmental transmission, captured by social networks and overlap in home ranges, respectively. Strikingly, microorganisms with distinct biological attributes drove these different transmission signals. While the social network effect on microbiota was driven by anaerobic bacteria, the effect of shared space was most influenced by aerotolerant spore-forming bacteria. These findings support the prediction that social contact is important for the transfer of microorganisms with low oxygen tolerance, while those that can tolerate oxygen or form spores may be able to transmit indirectly through the environment. Overall, these results suggest social and environmental transmission routes can spread biologically distinct members of the mammalian gut microbiota.
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