Probing the role of methyl methacrylate release from spacer materials in induced membrane bone healing.
Probing the role of methyl methacrylate release from spacer materials in induced membrane bone healing.
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DOI：
10.1002/jor.25147
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发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
Yang YP
中科院分区：
文献类型：
--
作者：
Stahl A;Park YB;Park SH;Lin S;Pan CC;Kim S;Yang YP
在用于骨重建的诱导膜（IM）技术中，植入聚（甲基丙烯酸甲酯）（PMMA）垫片以诱导在缺损部位周围形成异物膜。膜的发育对于以后的骨移植成功是必不可少的，但是IM促进骨再生的机制仍然未知，间隔物组合物在膜的愈合潜力中起作用的方式也是未知的。本研究调查了沥滤的甲基丙烯酸甲酯（MMA）-PMMA的主要单体成分-对IM发展的影响。体外细胞培养发现MMA洗脱液对内皮细胞和间充质干细胞的增殖没有影响。对于体内分析，我们提出了一种流线型大鼠股骨模型，以有效地研究垫片特性对IM特性的影响。比较聚己内酯（PCL）、MMA洗脱PCL（高剂量PCL-MMA和低剂量PCL-MMA）和手术PMMA周围的膜形成，发现体内4周后所有组均被坚固的膜包裹，与周围肌肉和骨组织相比，成骨形态发生蛋白-2（BMP 2）和血管生成血管内皮生长因子（VEGF）的表达升高。IM组织中的生长因子定量发现组间无统计学显著差异。还对聚合物植入物周围的新骨生长、血管化和CD 163+巨噬细胞群体进行了定量;发现高剂量PCL-MMA周围的血管形成与单独的PCL相比显著减少。据我们所知，这些研究结果代表了第一次，结果已经获得了有关的特点，在PCL周围形成的膜在IM设置。
In the induced membrane (IM) technique for bone reconstruction, a poly(methyl methacrylate) (PMMA) spacer is implanted to induce formation of a foreign body membrane around the defect site. Membrane development is essential for later bone grafting success, yet the mechanism by which the IM promotes bone regeneration remains unknown, as are the ways that spacer composition plays a role in the membrane’s healing potential. This study investigated the impact of leached methyl methacrylate (MMA) – the major monomeric component of PMMA – on IM development. In vitro cell culture found MMA elution did not impact endothelial cell or mesenchymal stem cell proliferation. For in vivo analysis, we advanced a streamlined rat femoral model to efficiently study the influence of spacer properties on IM characteristics. Comparison of membrane formation around polycaprolactone (PCL), MMA-eluting PCL (high dose PCL-MMA and low dose PCL-MMA), and surgical PMMA revealed robust membranes enveloped all groups after 4 weeks in vivo, with elevated expression of osteogenic bone morphogenetic protein-2 (BMP2) and angiogenic vascular endothelial growth factor (VEGF) compared with the surrounding muscle and bone tissues. Growth factor quantitation in IM tissue found no statistically significant difference between groups. New bone growth, vascularization, and CD163+ macrophage populations surrounding the polymer implants were also quantified; and blood vessel formation around high dose PCL-MMA was found to be significantly decreased compared with PCL alone. To our knowledge, these findings represent the first time that results have been obtained about the characteristics of membranes formed around PCL in the IM setting.
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影响因子：
2.7
作者：
Bruyas A;Lou F;Stahl AM;Gardner M;Maloney W;Goodman S;Yang YP
通讯作者：
Yang YP
影响因子：
14
作者：
Maruyama M;Nabeshima A;Pan CC;Behn AW;Thio T;Lin T;Pajarinen J;Kawai T;Takagi M;Goodman SB;Yang YP
通讯作者：
Yang YP
影响因子：
2.8
作者：
McBride-Gagyi, Sarah;Toth, Zacharie;Nicolaou, Daemeon
通讯作者：
Nicolaou, Daemeon
影响因子：
4.6
作者：
TSUCHIYA, H;HOSHINO, Y;TAKAGI, N
通讯作者：
TAKAGI, N
影响因子：
2.3
作者：
Masquelet, Alain C.
通讯作者：
Masquelet, Alain C.