The Arabidopsis general transcription factor TFIIB1 (AtTFIIB1) is required for pollen tube growth and endosperm development.
The Arabidopsis general transcription factor TFIIB1 (AtTFIIB1) is required for pollen tube growth and endosperm development.
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拟南芥通用转录因子 TFIIB1 (AtTFIIB1) 是花粉管生长和胚乳发育所必需的。
DOI：
10.1093/jxb/ert078
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发表时间：
2013-05
影响因子：
6.9
通讯作者：
Ye D
中科院分区：
文献类型：
--
作者：
Zhou JJ;Liang Y;Niu QK;Chen LQ;Zhang XQ;Ye D
花粉管生长和胚乳发育对受精和种子形成具有重要意义。这一过程的遗传机制仍然知之甚少。本研究报道了AtTFIIB1在花粉管生长和胚乳发育中的功能特征。AtTFIIB1与AtTFIIB2和AtTFIIB3/AtpBRP2的相似性分别为86%和44%。它在许多组织中表达，包括营养细胞核、花粉粒和花粉管的生殖细胞、胚乳和胚胎。这与AtTFIIB2和AtTFIIB3/AtpBRP2不同，前者在成熟花粉的胚乳和营养核中不表达，后者主要在雄性配子体中表达，而在种子中表达较弱。AtTFIIB1突变导致花粉管生长和胚乳发育严重迟缓，以及花粉管引导和接受受损，导致受精和种子发育中断。由AtTFIIB1启动子驱动的表达AtTFIIB2可以完全恢复有缺陷的花粉管生长、引导和接受，但只能部分恢复AtTFIIB1中的种子发育，而由AtTFIIB1启动子驱动的表达AtTFIIB3/AtpBRP2只能恢复有缺陷的AtTFIIB1种子。这些结果表明，AtTFIIB1在花粉管生长、引导、接受和胚乳发育中起重要作用，与AtTFIIB2和AtTFIIB3/AtpBRP2在功能上存在部分差异。
Pollen tube growth and endosperm development are important for fertilization and seed formation. The genetic mechanism of the processes remains poorly understood. This study reports the functional characterization of AtTFIIB1 in pollen tube growth and endosperm development. AtTFIIB1 shares 86% and 44% similarity with AtTFIIB2 and AtTFIIB3/AtpBRP2, respectively. It is expressed in many tissues including vegetative nuclei and generative cells of pollen grains and pollen tubes, endosperm, and embryos. It is thus different from AtTFIIB2, whose expression is not found in the endosperm and vegetative nucleus of mature pollen, and AtTFIIB3/AtpBRP2, which is expressed mostly in male gametophytes and weakly in seeds. Mutations in AtTFIIB1 caused a drastic retardation of pollen tube growth and endosperm development, as well as impaired pollen tube guidance and reception, leading to disruption of fertilization and seed development. Expression of AtTFIIB2 driven by the AtTFIIB1 promoter could restore the defective pollen tube growth, guidance, and reception completely, but only partially recovered the seed development in attfiib1, whilst expression of AtTFIIB3/AtpBRP2 driven by the AtTFIIB1 promoter could rescue only the defective attfiib1 seeds. All these results suggest that AtTFIIB1 plays important roles in pollen tube growth, guidance, and reception as well as endosperm development and is partially functionally different from AtTFIIB2 and AtTFIIB3/AtpBRP2.
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影响因子：
2.9
作者：
Kawashima T;Berger F
通讯作者：
Berger F
影响因子：
3.7
作者：
Cavel E;Pillot M;Pontier D;Lahmy S;Bies-Etheve N;Vega D;Grimanelli D;Lagrange T
通讯作者：
Lagrange T
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
12.3
作者：
Honys D;Twell D
通讯作者：
Twell D
影响因子：
64.5
作者：
Dieci, G;Sentenac, A
通讯作者：
Sentenac, A