Phase holograms for the three-dimensional patterning of unconstrained microparticles.
Phase holograms for the three-dimensional patterning of unconstrained microparticles.
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DOI：
10.1038/s41598-023-35337-8
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发表时间：
2023-06-06
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
声辐射力可以远程操纵粒子。来自驻波场的力使微尺度颗粒沿着场的波节或反波节位置对准以形成三维（3D）图案。这些图案可用于形成用于组织工程应用的3D微结构。然而，驻波产生需要多于一个换能器或反射器，这在体内实施是具有挑战性的。在这里，开发和验证的方法来操纵微球使用行波从一个单一的换能器。利用衍射理论和迭代角谱方法设计了相位全息图来整形声场。该场复制驻波并在压力节点处对准水中的聚乙烯微球，其类似于体内的细胞。使用戈尔'kov势来计算微球上的辐射力，轴向力被最小化，并且横向力被最大化以创建稳定的颗粒图案。来自相位全息图的压力场和所得到的颗粒聚集模式与特征相似性指数> 0.92的预测相匹配，其中1是完美匹配。产生的辐射力与驻波产生的辐射力相当，这表明有机会在体内实施细胞图案化以实现组织工程应用。
Acoustic radiation forces can remotely manipulate particles. Forces from a standing wave field align microscale particles along the nodal or anti-nodal locations of the field to form three-dimensional (3D) patterns. These patterns can be used to form 3D microstructures for tissue engineering applications. However, standing wave generation requires more than one transducer or a reflector, which is challenging to implement in vivo. Here, a method is developed and validated to manipulate microspheres using a travelling wave from a single transducer. Diffraction theory and an iterative angular spectrum approach are employed to design phase holograms to shape the acoustic field. The field replicates a standing wave and aligns polyethylene microspheres in water, which are analogous to cells in vivo, at pressure nodes. Using Gor’kov potential to calculate the radiation forces on the microspheres, axial forces are minimized, and transverse forces are maximized to create stable particle patterns. Pressure fields from the phase holograms and resulting particle aggregation patterns match predictions with a feature similarity index > 0.92, where 1 is a perfect match. The resulting radiation forces are comparable to those produced from a standing wave, which suggests opportunities for in vivo implementation of cell patterning toward tissue engineering applications.
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影响因子：
17.1
作者：
Gu Y;Chen C;Rufo J;Shen C;Wang Z;Huang PH;Fu H;Zhang P;Cummer SA;Tian Z;Huang TJ
通讯作者：
Huang TJ
影响因子：
4
作者：
Comeau, Eric S.;Hocking, Denise C.;Dalecki, Diane
通讯作者：
Dalecki, Diane
影响因子：
2.4
作者：
Garvin, Kelley A.;Dalecki, Diane;Hocking, Denise C.
通讯作者：
Hocking, Denise C.
影响因子：
2.9
作者：
Garvin, Kelley A.;Hocking, Denise C.;Dalecki, Diane
通讯作者：
Dalecki, Diane
DOI：
10.1073/pnas.2003569117
发表时间：
2020-07-07
影响因子：
11.1
作者：
Baresch, Diego;Garbin, Valeria
通讯作者：
Garbin, Valeria